Literature summary extracted from
Nguyen, T.T.; Jung, S.J.; Kang, H.K.; Kim, Y.M.; Moon, Y.H.; Kim, M.; Kim, D.
Production of rubusoside from stevioside by using a thermostable lactase from Thermus thermophilus and solubility enhancement of liquiritin and teniposide (2014), Enzyme Microb. Technol., 64-65, 38-43.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.21 |
expressed in Escherichia coli BL21(DE3)pLyS cells |
Thermus thermophilus |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.1.21 |
53000 |
- |
x * 53000, SDS-PAGE |
Thermus thermophilus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.21 |
Thermus thermophilus |
Q8GEB4 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.21 |
Ni-NTA column chromatography |
Thermus thermophilus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.21 |
stevioside + H2O |
- |
Thermus thermophilus |
rubusoside + D-glucose |
i.e. 13-O-beta-glucosyl-19-O-beta-D-glucosyl-steviol, the optimum rubusoside synthesis yield is 86% at 200 g/l with 1200 units lactase |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.21 |
? |
x * 53000, SDS-PAGE |
Thermus thermophilus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.21 |
beta-GLYPI |
- |
Thermus thermophilus |
3.2.1.21 |
lactase |
- |
Thermus thermophilus |