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Literature summary extracted from
- A thermostable S-adenosylhomocysteine hydrolase from Thermotoga maritima: properties and its application on S-adenosylhomocysteine production with enzymatic cofactor regeneration (2014), Enzyme Microb. Technol., 64-65, 33-37.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | expressed in Escherichia coli BL21(DE3) cells | Thermotoga maritima |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Thermotoga maritima | - |
L-homocysteine + adenosine | - |
? |  |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
L-homocysteine + adenosine | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.13.2.1 | Thermotoga maritima | O51933 | - |
- |
| 3.13.2.1 | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | O51933 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | immobilized nickel-affinity column chromatography, and gel filtration | Thermotoga maritima |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Thermotoga maritima | L-homocysteine + adenosine | - |
? | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | L-homocysteine + adenosine | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | S-adenosylhomocysteine hydrolase | - |
Thermotoga maritima |
| 3.13.2.1 | SAHase | - |
Thermotoga maritima |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 85 | - |
in the presence of 1 mM NAD+ | Thermotoga maritima |
| 3.13.2.1 | 100 | - |
in the presence of 8 mM NAD+ | Thermotoga maritima |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 8 | - |
in the presence of 1 mM NAD+ | Thermotoga maritima |
| 3.13.2.1 | 11.2 | - |
in the presence of 8 mM NAD+ | Thermotoga maritima |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.13.2.1 | NAD+ | - |
Thermotoga maritima | |
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