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Literature summary extracted from

  • Shinozaki, A.; Kawakami, T.; Hosokawa, S.; Sakamoto, T.
    A novel GH43 alpha-L-arabinofuranosidase of Penicillium chrysogenum that preferentially degrades single-substituted arabinosyl side chains in arabinan (2014), Enzyme Microb. Technol., 58-59, 80-86.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 gene afq1, semi-quantitative RT-PCR expression analysis, recombinant expression in and secretion from Escherichia coli Talaromyces funiculosus
3.2.1.55 gene afs1, semi-quantitative RT-PCR expression analysis, recombinant expression in and secretion from Escherichia coli Penicillium chrysogenum
3.2.1.55 gene Pcabf43A, semi-quantitative RT-PCR expression analysis Talaromyces funiculosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 additional information
-
additional information with L-arabinan, 0.5 mg/ml calculated as mM L-arabinofuranose released, pH 5.0, 50°C Penicillium chrysogenum
3.2.1.55 1.8
-
L-arabinan pH 5.0, 35°C Talaromyces funiculosus
3.2.1.55 11.5
-
L-arabinan pH 5.0, 50°C Talaromyces funiculosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.55 extracellular the isozyme is secreted Penicillium chrysogenum
-
-
3.2.1.55 extracellular the isozyme is secreted Talaromyces funiculosus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 33148
-
x * 33148 , isozyme PcABF43A, sequence calcualtion Talaromyces funiculosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Penicillium chrysogenum B5MGR1 inducible gene afs1 encoding isozyme AFS1
-
3.2.1.55 Penicillium chrysogenum 31B B5MGR1 inducible gene afs1 encoding isozyme AFS1
-
3.2.1.55 Talaromyces funiculosus B5MGR0 constitutive gene afq1 encoding isozyme AFQ1
-
3.2.1.55 Talaromyces funiculosus L0N6Q8 constitutive isozyme PcABF43A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 native extracellular enzyme 7.7fold from Penicillium funiculosum culture medium supernatant by ultrafiltration, ammonium sulfate fractionation, dialysis, two different steps of anion exchange chromatography, and hydrophobic interaction chromatography, to homogeneity Talaromyces funiculosus
3.2.1.55 recombinant isozyme AFQ1 from Escherichia coli culture supernatant Talaromyces funiculosus
3.2.1.55 recombinant isozyme AFS1 from Escherichia coli culture supernatant Penicillium chrysogenum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 1
-
purified enzyme, pH 5.0, 35°C, substrate L-arabinan Talaromyces funiculosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 1,5-alpha-L-arabinofuranobiose + H2O
-
Talaromyces funiculosus ?
-
?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Penicillium chrysogenum 4-nitrophenol + L-arabinofuranose
-
?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Talaromyces funiculosus 4-nitrophenol + L-arabinofuranose
-
?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Penicillium chrysogenum 31B 4-nitrophenol + L-arabinofuranose
-
?
3.2.1.55 alpha-(1->5)-L-arabinofuranotriose + H2O branched and unbranched, feruloylation of branched substrate strongly decreases the enzyme activity of isozyme rAFQ1 Talaromyces funiculosus ?
-
?
3.2.1.55 alpha-L-Araf-(1->3)-[alpha-L-Araf-(1->5)]-alpha-L-Araf + H2O branched, the activity of isozyme rAFS1on feruloylated branched L-arabinofuranotriose is almost the same as its activity on branched substrate Penicillium chrysogenum ?
-
?
3.2.1.55 alpha-L-Araf-(1->3)-[alpha-L-Araf-(1->5)]-alpha-L-Araf + H2O branched, the activity of isozyme rAFS1on feruloylated branched L-arabinofuranotriose is almost the same as its activity on branched substrate Penicillium chrysogenum 31B ?
-
?
3.2.1.55 L-arabinan + H2O
-
Talaromyces funiculosus ?
-
?
3.2.1.55 L-arabinan + H2O isozyme PcABF43A hydrolyzes arabinosyl side chains linked to C-2 or C-3 of single-substituted arabinose residues in L-arabinan, mode of action, overview Talaromyces funiculosus ?
-
?
3.2.1.55 L-arabinan + H2O mode of action, overview Penicillium chrysogenum ?
-
?
3.2.1.55 L-arabinan + H2O mode of action, overview Penicillium chrysogenum 31B ?
-
?
3.2.1.55 additional information activity of isozyme PcABF43A is strong and specific on sugar beet L-arabinan but weak on debranched arabinan andarabinoxylan. No or poor activity on 4-nitrophenyl alpha-L-arabinofuranoside, alpha-1,5-L-arabinooligosaccharides, and branched arabinotriose Talaromyces funiculosus ?
-
?
3.2.1.55 additional information no activity with unbranched L-arabinofuranotriose and 1,5-alpha-L-arabinofuranobiose Penicillium chrysogenum ?
-
?
3.2.1.55 additional information no activity with unbranched L-arabinofuranotriose and 1,5-alpha-L-arabinofuranobiose Penicillium chrysogenum 31B ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 ? x * 33148 , isozyme PcABF43A, sequence calcualtion Talaromyces funiculosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 ABF
-
Penicillium chrysogenum
3.2.1.55 ABF
-
Talaromyces funiculosus
3.2.1.55 AFQ1
-
Talaromyces funiculosus
3.2.1.55 AFS1
-
Penicillium chrysogenum
3.2.1.55 alpha-L-Afase
-
Penicillium chrysogenum
3.2.1.55 alpha-L-Afase
-
Talaromyces funiculosus
3.2.1.55 alpha-L-arabinofuranosidase
-
Penicillium chrysogenum
3.2.1.55 alpha-L-arabinofuranosidase
-
Talaromyces funiculosus
3.2.1.55 PcABF43A
-
Talaromyces funiculosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 35
-
isozyme PcABF43A Talaromyces funiculosus
3.2.1.55 50
-
isozyme AFQ1 Talaromyces funiculosus
3.2.1.55 50
-
isozyme AFS1 Penicillium chrysogenum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 30
-
purified native enzyme, pH 5.0, 1 h, more than 90% of the initial activity remains Talaromyces funiculosus
3.2.1.55 40
-
purified native enzyme, pH 5.0, 1 h, loss of 70% activity Talaromyces funiculosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5
-
isozyme AFQ1 Talaromyces funiculosus
3.2.1.55 5
-
isozyme AFS1 Penicillium chrysogenum
3.2.1.55 5
-
isozyme PcABF43A Talaromyces funiculosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.55 4 9 purified native enzyme, 16 h, more than 80% of the initial activity remains Talaromyces funiculosus
3.2.1.55 5
-
purified native enzyme, 30°C, 1 h, more than 90% of the initial activity remains Talaromyces funiculosus

Expression

EC Number Organism Comment Expression
3.2.1.55 Talaromyces funiculosus isozyme Pcabf43A gene is constitutively expressed in Penicillium chrysogenum 31B at a low level, although the expression is induced with pectic components such as L-arabinose, L-rhamnose, and D-galacturonic acid up
3.2.1.55 Penicillium chrysogenum slightly induced by L-rhamnose, D-galacturonic acid, rhamnogalacturonan, and polygalacturonic acid up
3.2.1.55 Talaromyces funiculosus slightly induced by L-rhamnose, D-galacturonic acid, rhamnogalacturonan, and polygalacturonic acid up

General Information

EC Number General Information Comment Organism
3.2.1.55 evolution isozyme AFQ1 belongs to the family 51 glycoside hydrolase, GH51 Talaromyces funiculosus
3.2.1.55 evolution isozyme AFS1 belongs to the family 54 glycoside hydrolase, GH54 Penicillium chrysogenum
3.2.1.55 additional information comparison of isozyme gene expression profiles Penicillium chrysogenum
3.2.1.55 additional information comparison of isozyme gene expression profiles Talaromyces funiculosus
3.2.1.55 physiological function isozyme AFQ1 plays a role of saccharification in the degradation of L-arabinan by this fungus Talaromyces funiculosus
3.2.1.55 physiological function isozyme PcABF43A together with isozyme AFS1 function as debranching enzymes in this fungus Penicillium chrysogenum
3.2.1.55 physiological function isozyme PcABF43A together with isozyme AFS1 function as debranching enzymes in this fungus Talaromyces funiculosus