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Literature summary extracted from

  • Weghoff, M.C.; Bertsch, J.; Müller, V.
    A novel mode of lactate metabolism in strictly anaerobic bacteria (2015), Environ. Microbiol., 17, 670-677.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.110 CaCl2 divalent cations stimulate activity, highest stimulation observed with CaCl2 is twofold. Activity increased linearly with increasing CaCl2 concentration and reaches saturation at 40 mM Acetobacterium woodii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.110 29000
-
 1 * 51000 (D-lactate dehydrogenase glycolate oxidase subunit GlcD) + 1 * 46000 (electron transfer flavoprotein alpha subunit) + 1 * 29000 (electron transfer flavoprotein beta), SDS-PAGE Acetobacterium woodii
1.3.1.110 46000
-
 1 * 51000 (D-lactate dehydrogenase glycolate oxidase subunit GlcD) + 1 * 46000 (electron transfer flavoprotein alpha subunit) + 1 * 29000 (electron transfer flavoprotein beta), SDS-PAGE Acetobacterium woodii
1.3.1.110 51000
-
 1 * 51000 (D-lactate dehydrogenase glycolate oxidase subunit GlcD) + 1 * 46000 (electron transfer flavoprotein alpha subunit) + 1 * 29000 (electron transfer flavoprotein beta), SDS-PAGE Acetobacterium woodii
1.3.1.110 138000
-
 gel filtration Acetobacterium woodii
1.3.1.110 151000
-
 native gel electrophoresis Acetobacterium woodii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.110 lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster Acetobacterium woodii lactate is a common substrate for major groups of strictly anaerobic bacteria. The LDH/Etf complex of Acetobacterium woodii uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+ pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
 r
1.3.1.110 lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster Acetobacterium woodii DSM 1030 lactate is a common substrate for major groups of strictly anaerobic bacteria. The LDH/Etf complex of Acetobacterium woodii uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+ pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.110 Acetobacterium woodii H6LBB0 and H6LBB1 and H6LBS1 H6LBB0: electron transfer flavoprotein beta subunit, H6LBB1: electron transfer flavoprotein alpha subunit, H6LBS1: D-lactate dehydrogenase subunit GlcD
-
1.3.1.110 Acetobacterium woodii DSM 1030 H6LBB0 and H6LBB1 and H6LBS1 H6LBB0: electron transfer flavoprotein beta subunit, H6LBB1: electron transfer flavoprotein alpha subunit, H6LBS1: D-lactate dehydrogenase subunit GlcD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.110
-
 Acetobacterium woodii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.110 lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster lactate is a common substrate for major groups of strictly anaerobic bacteria. The LDH/Etf complex of Acetobacterium woodii uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+ Acetobacterium woodii pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
 r
1.3.1.110 lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster pyruvate reduction to lactate with NADH as electron donor is highly exergonic Acetobacterium woodii pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
 r
1.3.1.110 lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster lactate is a common substrate for major groups of strictly anaerobic bacteria. The LDH/Etf complex of Acetobacterium woodii uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+ Acetobacterium woodii DSM 1030 pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
 r
1.3.1.110 lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster pyruvate reduction to lactate with NADH as electron donor is highly exergonic Acetobacterium woodii DSM 1030 pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
 r
1.3.1.110 pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster no NADH oxidation with pyruvate as electron acceptor. Only when oxidized ferredoxin is added to the system, the reaction is initiated and NADH oxidation is detected by a decrease of absorbance. NADH-dependent ferredoxin reduction is strictly dependent on the presence of lactate Acetobacterium woodii lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 r
1.3.1.110 pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster no NADH oxidation with pyruvate as electron acceptor. Only when oxidized ferredoxin is added to the system, the reaction is initiated and NADH oxidation is detected by a decrease of absorbance. NADH-dependent ferredoxin reduction is strictly dependent on the presence of lactate Acetobacterium woodii DSM 1030 lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 r

Subunits

EC Number Subunits Comment Organism
1.3.1.110 heterotrimer 1 * 51000 (D-lactate dehydrogenase glycolate oxidase subunit GlcD) + 1 * 46000 (electron transfer flavoprotein alpha subunit) + 1 * 29000 (electron transfer flavoprotein beta), SDS-PAGE Acetobacterium woodii

Synonyms

EC Number Synonyms Comment Organism
1.3.1.110 LDH/Etf complex
-
 Acetobacterium woodii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.110 20 30
-
 Acetobacterium woodii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.1.110 10 50 10°C: 65% of maximal activity, activity is optimal between 20 and 30°C. Activity above 30°C decreases by 20% and dropps down to only residual activity at 50°C Acetobacterium woodii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.110 7
-
-
 Acetobacterium woodii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.110 5.5 9 activity is rather insensitive to a pH ranging from 5.5 to 9.0 Acetobacterium woodii

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.110 FAD the LDH/Etf complex has three predicted FADs. FAD is required for stability and activity. FAD (0.005 mM) is present during all purification steps, as the enzyme precipitates in the absence of FAD. FMN can not substitute FAD Acetobacterium woodii
1.3.1.110 [4Fe-4S]-center the LDH/Etf complex has one predicted 4Fe-4S cluster Acetobacterium woodii

General Information

EC Number General Information Comment Organism
1.3.1.110 metabolism lactate is a common substrate for major groups of strictly anaerobic bacteria. The LDH/Etf complex of Acetobacterium woodii uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+ Acetobacterium woodii