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Literature summary extracted from

  • Zhou, L.; Ruan, M.; Cai, Q.; Liu, G.; Sun, L.; Su, W.; Cao, M.
    Purification, characterization and cDNA cloning of a trypsin from the hepatopancreas of snakehead (Channa argus) (2012), Comp. Biochem. Physiol. B, 161, 247-254.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 benzamidine 5 mM, 17.9% residual activity Channa argus
3.4.21.4 E-64 0.02 mM, 19.5% residual activity Channa argus
3.4.21.4 Lima bean trypsin inhibitor 0.002 mM, 7.9% residual activity Channa argus
3.4.21.4 mung bean trypsin inhibitor 0.003 mM, 3.6% residual activity Channa argus
3.4.21.4 Pefabloc SC 1 mM, 4.5% residual activity Channa argus
3.4.21.4 phenylmethylsulfonyl fluoride 10 mM, 4.5% residual activity Channa argus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.4 0.00049
-
 benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
3.4.21.4 0.00059
-
 benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
3.4.21.4 0.00064
-
 benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
3.4.21.4 0.00092
-
 benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
3.4.21.4 0.00102
-
 benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.4 Ca2+ 1 mM, 114% of initial activity Channa argus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.4 22000
-
 x * 22000, SDS-PAGE Channa argus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Channa argus G3LUK8
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.4 proteolytic modification sequence contains a signal peptide of 15 residues and an activation peptide of 9 amino acid residues with a mature protein of 223 residues Channa argus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.4
-
 Channa argus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.4 hepatopancreas
-
 Channa argus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.4 502
-
 pH 8.0, 37°C Channa argus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O 94.8% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.4 benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin + H2O 75.2% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Glu-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.4 benzyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O 14.8% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.4 benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O 51.4% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Leu-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.4 benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O 44.5% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Leu-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.4 benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
-
 Channa argus benzyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.4 benzyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O 27.1% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.4 benzyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O 33.5% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin Channa argus benzyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.4 additional information no substrates: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, Lys-7-amido-4-methylcoumarin and Arg-7-amido-4-methylcoumarin Channa argus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.4 ? x * 22000, SDS-PAGE Channa argus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.4 40
-
-
 Channa argus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.4 45
-
 stable below Channa argus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.4 72
-
 benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
3.4.21.4 76
-
 benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
3.4.21.4 80
-
 benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
3.4.21.4 92
-
 benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
3.4.21.4 148
-
 benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.4 9
-
-
 Channa argus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.4 7.5 9.5
-
 Channa argus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.4 87200
-
 benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
3.4.21.4 122200
-
 benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
3.4.21.4 143300
-
 benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
3.4.21.4 144700
-
 benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus
3.4.21.4 153900
-
 benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C Channa argus