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Literature summary extracted from
- Improved activity of alpha-chymotrypsin in mixed micelles of cetyltrimethylammonium bromide (CTAB) and ionic liquids: a kinetic study resorting to sequential injection analysis (2014), Colloids Surf. B Biointerfaces, 118, 172-178.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.1 | 0.024 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication | Bos taurus |  |
| 3.4.21.1 | 0.044 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 0.062 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 0.065 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 0.109 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 0.254 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 0.303 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 0.359 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 0.375 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication | Bos taurus | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.4.21.1 | additional information | kinetic evaluation of alpha-chymotrypsin activity in cetyltrimethylammonium bromide/ionic liquid mixed micelles and development of an automatic methodology, based on sequential injection analysis. cetyltrimethylammonium bromide/ionic liquids mixed micellar systems can induce alpha-chymotrypsin superactivity. CMC and average micellar size of cetyltrimethylammonium bromide/1-hexyl-3-methylimidazolium chloride, cetyltrimethylammonium bromide/1-butyl-3-methylimidazoliumchloride, cetyltrimethylammonium bromide/1-butyl-1-methylpyrrolidinium and cetyltrimethylammonium bromide/1-butyl-4-methylpyridinium chloride mixed micelles were evaluated by fluorescence and dynamic light scattering, respectively. The sequential injection analysis methodology is suitable for evaluation of alpha-chymotrypsin activity in mixed micelles as it proves to be robust and exhibits good repeatability in all the assay conditions | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.1 | Bos taurus | P00766 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.1 | commercial preparation | - |
Bos taurus | - |
| 3.4.21.1 | pancreas | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.1 | N-succinyl-L-phenylalanine-4-nitroanilide + H2O | - |
Bos taurus | N-succinyl-L-phenylalanine + 4-nitroaniline | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.1 | 0.154 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 0.236 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 0.362 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 0.417 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 0.48 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 0.807 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 0.985 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 1.054 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 1.177 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication | Bos taurus | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.1 | 2.74 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 3.14 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 3.18 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 3.48 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 4.44 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 5.35 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 5.87 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 6.42 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
| 3.4.21.1 | 6.47 | - |
N-succinyl-L-phenylalanine-4-nitroanilide | presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication | Bos taurus | |
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