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Literature summary extracted from

  • Chen, H.; Yang, H.; Hu, Z.; Yang, H.; Ma, H.; Gao, S.; Guo, Q.; Bai, W.; Qin, L.; Li, L.
    Prokaryotic expression and activity analysis of 5'-methylthioadenosine nucleosidase in Mycobacterium tuberculosis (2012), Chin. J. Microbiol. Immunol., 32, 589-594.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.16 expressed in Escherichia coli BL21trxB cells Mycobacterium tuberculosis
3.2.2.16 gene RV0091, recombinant expression of active His-tagged enzyme in Escherichia coli strain BL21trxB, optimization of expression method Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.16 S-methyl-5'-thioadenosine + H2O Mycobacterium tuberculosis
-
 S-methyl-5-thio-D-ribose + adenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.16 Mycobacterium tuberculosis
-
-
-
3.2.2.16 Mycobacterium tuberculosis P9WJN3 gene RV0091
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.16 Ni-NTA column chromatography, and gel filtration Mycobacterium tuberculosis
3.2.2.16 recombinant His-tagged enzyme from Escherichia coli strain BL21trxB by nickel affinity chromatography to over 95% purity Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.16 5'-methylthioadenosine + H2O
-
 Mycobacterium tuberculosis adenine + S-methyl-5-thio-D-ribose
-
 ?
3.2.2.16 S-methyl-5'-thioadenosine + H2O
-
 Mycobacterium tuberculosis S-methyl-5-thio-D-ribose + adenine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.16 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Mycobacterium tuberculosis
3.2.2.16 5-methylthioadenosine nucleosidase
-
 Mycobacterium tuberculosis
3.2.2.16 MTAN
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.16 37
-
-
 Mycobacterium tuberculosis
3.2.2.16 37
-
 recombinant His-tagged enzyme Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.16 additional information
-
 the purified recombinant His-tagged enzyme shows a poor thermal stability Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.16 10 12
-
 Mycobacterium tuberculosis
3.2.2.16 10 12 recombinant His-tagged enzyme Mycobacterium tuberculosis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.2.16 7.5 12 recombinant His-tagged enzyme, stable at, denaturation and loss of activity at and below pH 7.0 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.2.2.16 metabolism the enzyme plays a key role in the metabolism of Mycobacterium tuberculosis Mycobacterium tuberculosis