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Literature summary extracted from

  • Silipo, A.; Larsbrink, J.; Marchetti, R.; Lanzetta, R.; Brumer, H.; Molinaro, A.
    NMR spectroscopic analysis reveals extensive binding interactions of complex xyloglucan oligosaccharides with the Cellvibrio japonicus glycoside hydrolase family 31 alpha-xylosidase (2012), Chemistry, 18, 13395-13404.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.177 D659A site-directed mutagenesis, active site mutant, catalytically inactive variant Cellvibrio japonicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.177 alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O Cellvibrio japonicus
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 D-xylose + beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4) D-Glc
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Organism

EC Number Organism UniProt Comment Textmining
3.2.1.177 Cellvibrio japonicus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.177 alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
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 Cellvibrio japonicus D-xylose + beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4) D-Glc
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3.2.1.177 additional information the enzyme CjXyl31A demonstrates an exquisite regioselectivity for the terminal nonreducing-end alpha-(1->6)-xylose of xyloglucan oliogosaccharides Cellvibrio japonicus ?
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3.2.1.177 xyloglucan oligosaccharide + H2O extensive interactions between the enzyme's active-site variants and xyloglucan hexa- and heptasaccharides. The enzyme recognizes the entire cello-tetraosyl backbone of the substrate and product in positive enzyme subsites and makes further significant interactions with internal pendant alpha-(1->6)-linked xylosyl units, role of the PA14 domain, stereochemistry of the hydrolysis, overview Cellvibrio japonicus ?
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Synonyms

EC Number Synonyms Comment Organism
3.2.1.177 alpha-xylosidase
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 Cellvibrio japonicus
3.2.1.177 alpha-xylosidase 31A
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 Cellvibrio japonicus
3.2.1.177 CjXyl31A
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 Cellvibrio japonicus

General Information

EC Number General Information Comment Organism
3.2.1.177 evolution the enzyme belongs to the glycoside hydrolase family 31, GH31 Cellvibrio japonicus
3.2.1.177 additional information extensive interactions between the enzyme's active-site variants and xyloglucan hexa- and heptasaccharides, role of the PA14 domain, structure-function analysis using a combination of NMR spectroscopic techniques, including saturation transfer difference and transfer NOE spectroscopy Cellvibrio japonicus