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Literature summary extracted from

  • Xu, Y.; Zhou, X.; Bai, Y.; Wang, J.; Wu, C.; Xu, X.; Jin, Z.
    Cycloamylose production from amylomaize by isoamylase and Thermus aquaticus 4-alpha-glucanotransferase (2014), Carbohydr. Polym., 102, 66-73.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.68 synthesis the enzyme is used for debranching of amylomaize in the production of cycloamylose, natural amylopectin containing starch with enhanced conversion yield after debranching, use of Thermus aquaticus 4-alpha-glucanotransferase for conversion of debranched amylomaize amylose and amylomaize amylopectin into cycloamylose, overview Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 expressed in Escherichia coli Thermus aquaticus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Thermus aquaticus
-
-
-
3.2.1.68 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25 Ni-NTA column chromatography Thermus aquaticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.68 commercial preparation
-
 Pseudomonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 amylomaize V + glycosyl acceptor
-
 Thermus aquaticus cycloamylose + ?
-
 ?
2.4.1.25 potato amylose + glycosyl acceptor
-
 Thermus aquaticus cycloamylose + ?
-
 ?
3.2.1.68 amylomaize + H2O debranching Pseudomonas sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 4alphaGTase
-
 Thermus aquaticus