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Literature summary extracted from

  • Liao, H.; Li, S.; Zheng, H.; Wei, Z.; Liu, D.; Raza, W.; Shen, Q.; Xu, Y.
    A new acidophilic thermostable endo-1,4-beta-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris (2014), BMC Biotechnol., 14, 90.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 gene poman5A, recombinant expression of C-terminally His6-tagged extracellular enzyme in and secretion from Pichia pastoris strain GS115 Penicillium oxalicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Fe3+
-
 Penicillium oxalicum
3.2.1.78 Hg2+
-
 Penicillium oxalicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 additional information
-
 additional information Michaelis-Menten kinetics, Km values for locust bean gum, konjac mannan, and guar gum are 7.6 mg/ml, 2.1 mg/ml, and 2.3 mg/ml, respectively, pH 4.0, 80°C Penicillium oxalicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.78 extracellular the enzyme is secreted Penicillium oxalicum
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.78 additional information Penicillium oxalicum the enzyme catalyzes the random hydrolysis of beta-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans, main products are mannobiose, mannotriose, and mannopentaose ?
-
 ?
3.2.1.78 additional information Penicillium oxalicum GZ-2 the enzyme catalyzes the random hydrolysis of beta-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans, main products are mannobiose, mannotriose, and mannopentaose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Penicillium oxalicum
-
 gene poman5A
-
3.2.1.78 Penicillium oxalicum GZ-2
-
 gene poman5A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 recombinant His6-tagged extracellular enzyme from Pichia pastoris strain GS115 culture supernatant by nickel affinity chromatography to homogeneity Penicillium oxalicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 additional information
-
 84.4 U/ml of recombinant enzyme in the culture supernatant of Pichia pastoris, pH 4.0, 80°C Penicillium oxalicum
3.2.1.78 420.9
-
 purified recombinant enzyme, locust bean gum as substrate, pH 4.0, 80°C Penicillium oxalicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 guar gum + H2O
-
 Penicillium oxalicum ?
-
 ?
3.2.1.78 guar gum + H2O
-
 Penicillium oxalicum GZ-2 ?
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Penicillium oxalicum ?
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Penicillium oxalicum GZ-2 ?
-
 ?
3.2.1.78 additional information the enzyme catalyzes the random hydrolysis of beta-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans, main products are mannobiose, mannotriose, and mannopentaose Penicillium oxalicum ?
-
 ?
3.2.1.78 additional information the enzyme catalyzes the random hydrolysis of beta-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans, main products are mannobiose, mannotriose, and mannopentaose Penicillium oxalicum GZ-2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 endo-1,4-beta-mannanase
-
 Penicillium oxalicum
3.2.1.78 PoMan5A
-
 Penicillium oxalicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 80
-
 recombinant enzyme Penicillium oxalicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 60
-
 purified recombinant His6-tagged enzyme, half-life is approximately 58 h at pH 4.0 Penicillium oxalicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 4
-
 recombinant enzyme Penicillium oxalicum

General Information

EC Number General Information Comment Organism
3.2.1.78 evolution the enzyme belongs to the glycosyl hydrolase family 5, GH5 Penicillium oxalicum