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Literature summary extracted from

  • Shi, H.; Ding, H.; Huang, Y.; Wang, L.; Zhang, Y.; Li, X.; Wang, F.
    Expression and characterization of a GH43 endo-arabinanase from Thermotoga thermarum (2014), BMC Biotechnol., 14, 35.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.99 SDS 111% activity at 0.1% SDS Pseudothermotoga thermarum
3.2.1.99 Tween 60 133% activity at 0.05% Tween 60 Pseudothermotoga thermarum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.99 expressed in Escherichia coli BL21(DE3) cells Pseudothermotoga thermarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.99 Al3+ complete inhibition at 1 mM Pseudothermotoga thermarum
3.2.1.99 Co2+ 82% residual activity at 1 mM Pseudothermotoga thermarum
3.2.1.99 Cu2+ complete inhibition at 1 mM Pseudothermotoga thermarum
3.2.1.99 Ni2+ 31% residual activity at 1 mM Pseudothermotoga thermarum
3.2.1.99 Tris 41% residual activity at 0.05% (w/v) Pseudothermotoga thermarum
3.2.1.99 Zn2+ 14% residual activity at 1 mM Pseudothermotoga thermarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.99 Ba2+ 113% activity at 1 mM Pseudothermotoga thermarum
3.2.1.99 Ca2+ 113% activity at 1 mM Pseudothermotoga thermarum
3.2.1.99 Mn2+ 128% activity at 1 mM Pseudothermotoga thermarum
3.2.1.99 additional information not affected by Mg2+ Pseudothermotoga thermarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.99 36000
-
 x * 36000, SDS-PAGE Pseudothermotoga thermarum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Pseudothermotoga thermarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.99 nickel affinity column chromatography Pseudothermotoga thermarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.99 42.5
-
 with sugar beet arabinan as substrate, at pH 6.5 and 75°C Pseudothermotoga thermarum
3.2.1.99 199.7
-
 with debranched arabinan as substrate, at pH 6.5 and 75°C Pseudothermotoga thermarum
3.2.1.99 237.7
-
 with linear arabinan as substrate, at pH 6.5 and 75°C Pseudothermotoga thermarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 debranched arabinan + H2O
-
 Pseudothermotoga thermarum oligo-alpha-(1->5)-L-arabinoside
-
 ?
3.2.1.99 linear arabinan + H2O
-
 Pseudothermotoga thermarum oligo-alpha-(1->5)-L-arabinoside
-
 ?
3.2.1.99 additional information there is no activity towards 1,4-beta-D-mannan, galactan, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-alpha-L-arabinofuranoside Pseudothermotoga thermarum ?
-
 ?
3.2.1.99 sugar beet arabinan + H2O
-
 Pseudothermotoga thermarum oligo-alpha-(1->5)-L-arabinoside
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.99 ? x * 36000, SDS-PAGE Pseudothermotoga thermarum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.99 ABN
-
 Pseudothermotoga thermarum
3.2.1.99 endo-arabinanase
-
 Pseudothermotoga thermarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.99 75
-
-
 Pseudothermotoga thermarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.99 60 90 about 50% activity at pH 60°C, about 70% activity at pH 70°C, about 95% activity at pH 80°C, about 92% activity at pH 85°C, about 20% activity at 90°C Pseudothermotoga thermarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.99 70 85 the enzyme retains more than 95% of its initial activity at 70°C for 1 h. After being incubated at 80°C for 2 h, the residual activity retained is more than 80% of the initial activity. The enzyme retains more than 90% of its maximum activity at 85°C after 10 min incubation Pseudothermotoga thermarum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.99 349.3
-
 linear arabinan at pH 6.5 and 75°C Pseudothermotoga thermarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.99 6.5
-
-
 Pseudothermotoga thermarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.99 5.5 7.5 about 35% activity at pH 5.5, about 50% activity at pH 7.5 Pseudothermotoga thermarum