EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.16.4 | synthesis | highest yield of protein expression and purification from 12 mg of protein per liter of culture at 3 l bioreactor-scale is achieved in Streptomyces venezuelae ATCC 10595, a fast growing Streptomyces susceptible to glycopeptides. The addition of His6-tag at the N-terminus of the protein abolishes its biological activity either in vitro or in vivo assays. The addition of His6-tag at the C-terminus of the protein leads to a functional protein | Nonomuraea sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.16.4 | expression in Streptomyces spp. | Nonomuraea sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.16.4 | additional information | the addition of His6-tag at the N-terminus of the protein abolishes its biological activity either in vitro or in vivo assays. The addition of His6-tag at the C-terminus of the protein leads to a functional protein | Nonomuraea sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.16.4 | cell wall | enzyme preferentially accumulates in the cell wall fractions obtained by the step of spheroplast preparation | Nonomuraea sp. | 5618 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.16.4 | 22100 | - |
x * 25000, SDS-PAGE of His-tagged protein, x * 22100, calculated for native protein | Nonomuraea sp. |
3.4.16.4 | 25000 | - |
x * 25000, SDS-PAGE of His-tagged protein, x * 22100, calculated for native protein | Nonomuraea sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.16.4 | Nonomuraea sp. | Q7WZ84 | - |
- |
3.4.16.4 | Nonomuraea sp. ATCC 39727 | Q7WZ84 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.16.4 | 3 | 8 | substrate Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala , pH 7.4, 25°C | Nonomuraea sp. |
3.4.16.4 | 18 | - |
substrate D-Ala-D-Ala, pH 7.4, 25°C | Nonomuraea sp. |
3.4.16.4 | 40 | - |
substrate acetyl-L-Lys-D-Ala-D-Ala, pH 7.4, 25°C | Nonomuraea sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.16.4 | acetyl-L-Lys-D-Ala-D-Ala + H2O | - |
Nonomuraea sp. | acetyl-L-Lys-D-Ala + D-Ala | - |
? | |
3.4.16.4 | acetyl-L-Lys-D-Ala-D-Ala + H2O | - |
Nonomuraea sp. ATCC 39727 | acetyl-L-Lys-D-Ala + D-Ala | - |
? | |
3.4.16.4 | D-Ala-D-Ala + H2O | - |
Nonomuraea sp. | D-Ala + D-Ala | - |
? | |
3.4.16.4 | D-Ala-D-Ala + H2O | - |
Nonomuraea sp. ATCC 39727 | D-Ala + D-Ala | - |
? | |
3.4.16.4 | Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O | - |
Nonomuraea sp. | Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala | - |
? | |
3.4.16.4 | Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O | - |
Nonomuraea sp. ATCC 39727 | Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.16.4 | ? | x * 25000, SDS-PAGE of His-tagged protein, x * 22100, calculated for native protein | Nonomuraea sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.16.4 | Dbv7 | - |
Nonomuraea sp. |
3.4.16.4 | VanYn | - |
Nonomuraea sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.16.4 | physiological function | enzyme has a role in removing the last D-Ala from the pentapeptide peptidoglycan precursors and reprogramming cell wall biosynthesis. Isoform C-His6-VanYn over-expression confers glycopeptide resistance to Streptomyces venezuelae. The addition of His6-tag at the N-terminus of the protein abolishes its biological activity either in vitro or in vivo assays | Nonomuraea sp. |