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Literature summary extracted from

  • Haq, I.U.; Khan, M.A.; Muneer, B.; Hussain, Z.; Afzal, S.; Majeed, S.; Rashid, N.; Javed, M.M.; Ahmad, I.
    Cloning, characterization and molecular docking of a highly thermostable beta-1,4-glucosidase from Thermotoga petrophila (2012), Biotechnol. Lett., 34, 1703-1709.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 gene bglA, recombinant expression in Escherichia coli strain BL21 Codon-Plus Thermotoga petrophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 1-propanol 40% v/v, almost complete inhibition Thermotoga petrophila
3.2.1.21 Cu2+ 20% inhibition at 1 mM Thermotoga petrophila
3.2.1.21 ethanol 40% v/v, almost complete inhibition Thermotoga petrophila
3.2.1.21 Hg2+ 90% inhibition at 1 mM Thermotoga petrophila
3.2.1.21 methanol 40% v/v, almost complete inhibition Thermotoga petrophila
3.2.1.21 additional information the enzyme is not inhibited by glucose up to 5% w/v Thermotoga petrophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 2.8
-
 4-nitrophenyl-beta-D-glucopyranoside pH 7.0, 90°C Thermotoga petrophila
3.2.1.21 4.3
-
 4-nitrophenyl-beta-D-cellobioside pH 7.0, 90°C Thermotoga petrophila
3.2.1.21 25
-
 4-nitrophenyl-beta-D-xylopyranoside pH 7.0, 90°C Thermotoga petrophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 51500
-
 x * 51500 SDS-PAGE Thermotoga petrophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Thermotoga petrophila A5IL97 gene bglA
-
3.2.1.21 Thermotoga petrophila RKU-1 A5IL97 gene bglA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 recombinant enzyme from Escherichia coli strain BL21 Codon-Plus Thermotoga petrophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 2.3
-
 purified recombinant enzyme, substrate cellobiose, pH 7.0, 90°C Thermotoga petrophila
3.2.1.21 1520
-
 purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 7.0, 90°C Thermotoga petrophila
3.2.1.21 9400
-
 purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-cellobioside, pH 7.0, 90°C Thermotoga petrophila
3.2.1.21 14000
-
 purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-galactopyranoside, pH 7.0, 90°C Thermotoga petrophila
3.2.1.21 29200
-
 purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-fucopyranoside, pH 7.0, 90°C Thermotoga petrophila
3.2.1.21 30400
-
 purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside, pH 7.0, 90°C Thermotoga petrophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-cellobioside + H2O
-
 Thermotoga petrophila 4-nitrophenol + cellobiose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-cellobioside + H2O
-
 Thermotoga petrophila RKU-1 4-nitrophenol + cellobiose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + beta-D-fucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Thermotoga petrophila RKU-1 4-nitrophenol + beta-D-fucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermotoga petrophila RKU-1 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermotoga petrophila RKU-1 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + D-xylopyranose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Thermotoga petrophila 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Thermotoga petrophila RKU-1 2 beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 ? x * 51500 SDS-PAGE Thermotoga petrophila

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 BglA
-
 Thermotoga petrophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 80 90
-
 Thermotoga petrophila

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 30 100 activity range, profile overview Thermotoga petrophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 5300
-
 4-nitrophenyl-beta-D-xylopyranoside pH 7.0, 90°C Thermotoga petrophila
3.2.1.21 17500
-
 4-nitrophenyl-beta-D-cellobioside pH 7.0, 90°C Thermotoga petrophila
3.2.1.21 87400
-
 4-nitrophenyl-beta-D-glucopyranoside pH 7.0, 90°C Thermotoga petrophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 7 8
-
 Thermotoga petrophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 4 10 activity range, profile overview Thermotoga petrophila

General Information

EC Number General Information Comment Organism
3.2.1.21 additional information enzyme-substrate docking studies, overview Thermotoga petrophila

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.21 210
-
 4-nitrophenyl-beta-D-xylopyranoside pH 7.0, 90°C Thermotoga petrophila
3.2.1.21 4100
-
 4-nitrophenyl-beta-D-cellobioside pH 7.0, 90°C Thermotoga petrophila
3.2.1.21 30800
-
 4-nitrophenyl-beta-D-glucopyranoside pH 7.0, 90°C Thermotoga petrophila