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Literature summary extracted from

  • Hakamada, Y.; Ohkubo, Y.; Ohashi, S.
    Purification and characterization of beta-mannanase from Reinekea sp. KIT-YO10 with transglycosylation activity (2014), Biosci. Biotechnol. Biochem., 78, 722-728.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 additional information Ca2+, Mg2+, Mn2+, Fe2+, Ni2+, and Zn2+ have either no effect or a slightly inhibitory effect Reinekea sp. KIT-YO10

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 additional information
-
 additional information Michaelis-Menten kinetics Reinekea sp. KIT-YO10

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 44300
-
 x * 44300, SDS-PAGE Reinekea sp. KIT-YO10

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.78 konjac glucomannan + H2O Reinekea sp. KIT-YO10
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Reinekea sp. KIT-YO10
-
 isolated from the seashore of Kanazawa Port in Japan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 native enzyme 29.4fold by anion exchange chromatography Reinekea sp. KIT-YO10

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 236
-
 pH 8.0, 40°C, substrate konjac glucomannan Reinekea sp. KIT-YO10

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 guar gum + H2O 1.7% activity compared to konjac glucomannan Reinekea sp. KIT-YO10 ?
-
 ?
3.2.1.78 ivory nut mannan + H2O 46% activity compared to konjac glucomannan Reinekea sp. KIT-YO10 ?
-
 ?
3.2.1.78 konjac glucomannan + H2O
-
 Reinekea sp. KIT-YO10 ?
-
 ?
3.2.1.78 konjac glucomannan + H2O best substrate Reinekea sp. KIT-YO10 ?
-
 ?
3.2.1.78 locust bean gum + H2O 9.6% activity compared to konjac glucomannan Reinekea sp. KIT-YO10 ?
-
 ?
3.2.1.78 additional information no activity with tamarind xyloglucan, sodium arginate, carboxymethylcellulose, low-melting point agarose, gellan gum, xylan, carrageenan, and laminaran Reinekea sp. KIT-YO10 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 44300, SDS-PAGE Reinekea sp. KIT-YO10

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 Beta-mannanase
-
 Reinekea sp. KIT-YO10
3.2.1.78 endo-1,4-beta-D-mannanase
-
 Reinekea sp. KIT-YO10
3.2.1.78 Rman
-
 Reinekea sp. KIT-YO10

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 70
-
-
 Reinekea sp. KIT-YO10

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.78 30 80 activity range, profile overview Reinekea sp. KIT-YO10

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 20 50 purified enzyme, pH 8.0, 20 min, stable at Reinekea sp. KIT-YO10
3.2.1.78 70
-
 purified enzyme, pH 8.0, 20 min, loss of 50% activity Reinekea sp. KIT-YO10
3.2.1.78 90
-
 purified enzyme, pH 8.0, 20 min, inactivation Reinekea sp. KIT-YO10

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 9
-
-
 Reinekea sp. KIT-YO10

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 7 9 over 80% of amximal activity Reinekea sp. KIT-YO10

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 8
-
 purified enzyme, 30°C, 30 min, over 90% activity remaining Reinekea sp. KIT-YO10