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Literature summary extracted from

  • Kobayashi, T.; Uchimura, K.; Koide, O.; Deguchi, S.; Horikoshi, K.
    Genetic and biochemical characterization of the Pseudoalteromonas tetraodonis alkaline kappa-carrageenase (2012), Biosci. Biotechnol. Biochem., 76, 506-511.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.83 DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain DH5alpha as extracellular enzyme Pseudoalteromonas tetraodonis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.83 EDTA
-
 Pseudoalteromonas tetraodonis
3.2.1.83 EGTA
-
 Pseudoalteromonas tetraodonis
3.2.1.83 additional information no effect by NEM, iodoacetate, 2-phenanthroline, DTT, 2-mercaptoethanol, N-bromosuccimide, and 4-chloromercuribenzoate Pseudoalteromonas tetraodonis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.83 extracellular the enzyme contains an N-terminal 28-amino acid signal peptide Pseudoalteromonas tetraodonis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.83 Ca2+ activates Pseudoalteromonas tetraodonis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.83 41809
-
 2 * 41809, sequence calculation Pseudoalteromonas tetraodonis
3.2.1.83 85000
-
 recombinant enzyme, gel filtration Pseudoalteromonas tetraodonis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.83 kappa-carrageen + H2O Pseudoalteromonas tetraodonis the enzyme cleaves in an endo-fashion ?
-
 ?
3.2.1.83 kappa-carrageen + H2O Pseudoalteromonas tetraodonis JAM-K142 the enzyme cleaves in an endo-fashion ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.83 Pseudoalteromonas tetraodonis E7FKF4
-
-
3.2.1.83 Pseudoalteromonas tetraodonis JAM-K142 E7FKF4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.83 recombinant extracelluar enzyme 388.6fold from Escherichia coli strain DH5alpha by ammonium sulfate fractionation, dialysis, anion and cation exchange chromatography to homogeneity Pseudoalteromonas tetraodonis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.83 8.16
-
 purified recombinant enzyme, pH 6.0, 50°C Pseudoalteromonas tetraodonis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.83 kappa-carrageen + H2O the enzyme cleaves in an endo-fashion Pseudoalteromonas tetraodonis ?
-
 ?
3.2.1.83 kappa-carrageen + H2O the enzyme cleaves in an endo-fashion Pseudoalteromonas tetraodonis JAM-K142 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.83 homodimer 2 * 41809, sequence calculation Pseudoalteromonas tetraodonis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.83 Cgk-K142
-
 Pseudoalteromonas tetraodonis
3.2.1.83 kappa-carrageenase-like protein
-
 Pseudoalteromonas tetraodonis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.83 55
-
 recombinant enzyme, pH 8.0 Pseudoalteromonas tetraodonis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.83 additional information
-
 no protective effect on enzyme thermal stability by Ca2+, Mg2+, agar, and NaCl Pseudoalteromonas tetraodonis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.83 8
-
 recombinant enzyme, at 50°C Pseudoalteromonas tetraodonis
3.2.1.83 8.8
-
 recombinant enzyme, at 30°C Pseudoalteromonas tetraodonis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.83 Pseudoalteromonas tetraodonis sequence calculation
-
 5.1