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Literature summary extracted from

  • Yuan, P.; Meng, K.; Wang, Y.; Luo, H.; Shi, P.; Huang, H.; Bai, Y.; Yang, P.; Yao, B.
    A protease-resistant exo-polygalacturonase from Klebsiella sp. Y1 with good activity and stability over a wide pH range in the digestive tract (2012), Biores. Technol., 123, 171-176.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.67 2-mercaptoethanol slight activation at 5 mM Klebsiella sp. Y1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.67 gene pgu B, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21DE3 Klebsiella sp. Y1

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 Ca2+ 83% inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 Co2+ 90% inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 Cr3+ 90% inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 Cu2+ complete inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 EDTA 8% inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 Fe3+ 40% inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 K+ 25% inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 Li+ 33% inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 Mg2+ 36% inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 Mn2+ 64% inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 Na+ 29% inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 Ni2+ 94% inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 Pb2+ 88% inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 SDS 83% inhibition at 5 mM Klebsiella sp. Y1
3.2.1.67 Zn2+ 90% inhibition at 5 mM Klebsiella sp. Y1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.67 additional information predicted presence of a putative signal peptide at residues 1-24 Klebsiella sp. Y1
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.67 72200
-
 x * 72200, about, sequence calculation Klebsiella sp. Y1

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Klebsiella sp. Y1 J9QQ32 gene pgu B
-
3.2.1.67 Klebsiella sp. Y1 CGMCC 4433 J9QQ32 gene pgu B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 recombinant His-tagged enzyme from Escherichia coli strain BL21DE3 by ncikel affinity chromatography Klebsiella sp. Y1

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.67 47
-
 purified recombinant His-tagged enzyme, substrate [(1->4)-alpha-D-galacturonide]n, pH 6.0, 40°C Klebsiella sp. Y1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 additional information the enzyme is a strict exo-type polygalacturonase and reduces the viscosity of polygalacturonic acid (1.0%, w/v) by 15.6% Klebsiella sp. Y1 ?
-
 ?
3.2.1.67 additional information the enzyme is a strict exo-type polygalacturonase and reduces the viscosity of polygalacturonic acid (1.0%, w/v) by 15.6% Klebsiella sp. Y1 CGMCC 4433 ?
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]n + H2O polygalacturonnic acid Klebsiella sp. Y1 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]n + H2O polygalacturonnic acid Klebsiella sp. Y1 CGMCC 4433 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 ? x * 72200, about, sequence calculation Klebsiella sp. Y1

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 exo-PGU B
-
 Klebsiella sp. Y1
3.2.1.67 exo-polygalacturonase
-
 Klebsiella sp. Y1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 40 50
-
 Klebsiella sp. Y1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.67
-
 70 activity range, recombinant enzyme Klebsiella sp. Y1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 50 60 purified recombinant His-tagged enzyme, pH 6.0, 5-10 min, stable at 50°C, loss of over 80% activity at 60°C Klebsiella sp. Y1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 6
-
-
 Klebsiella sp. Y1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.67 2 12 35% of maximal ativity within this range Klebsiella sp. Y1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.67 1.5 6.8 under simulated alimentary tract conditions, the enzyme is very stable and active showing over 25% activity from pH 1.5 to pH 6.8 Klebsiella sp. Y1
3.2.1.67 2 12 purified recombinant His-tagged enzyme, 37°C, 1 h, over 30% activity remaining Klebsiella sp. Y1
3.2.1.67 3 9 purified recombinant His-tagged enzyme, 37°C, 1 h, over 65% activity remaining Klebsiella sp. Y1

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.67 Klebsiella sp. Y1 sequence calculation
-
 5.5

General Information

EC Number General Information Comment Organism
3.2.1.67 evolution the enzyme belongs to the glycosyl hydrolase 28 family, GH28 Klebsiella sp. Y1