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Literature summary extracted from

  • Deng, L.; Tsybina, P.; Gregg, K.; Mosi, R.; Zandberg, W.; Boraston, A.; Vocadlo, D.
    Synthesis of 4-methylumbelliferyl alpha-D-mannopyranosyl-(1-6)-beta-D-mannopyranoside and development of a coupled fluorescent assay for GH125 exo-alpha-1,6-mannosidases (2013), Bioorg. Med. Chem., 21, 4839-4845.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.163 0.38
-
 4-methylumbelliferyl alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranoside pH 7.4, 37°C Clostridium perfringens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.163 Clostridium perfringens
-
-
-
3.2.1.163 Streptococcus pneumoniae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.163 4-methylumbelliferyl alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranoside + H2O synthesis of the fluorogenic disaccharide substrate and assay method development, overview Streptococcus pneumoniae 4-methylumbelliferone + alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranose
-
 ?
3.2.1.163 4-methylumbelliferyl alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranoside + H2O synthesis of the fluorogenic disaccharide substrate and assay method development, overview Clostridium perfringens 4-methylumbelliferone + alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.163 CpGH125
-
 Clostridium perfringens
3.2.1.163 GH125 exo-alpha-1,6-mannosidase
-
 Streptococcus pneumoniae
3.2.1.163 GH125 exo-alpha-1,6-mannosidase
-
 Clostridium perfringens
3.2.1.163 SpGH125
-
 Streptococcus pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.163 37
-
 assay at Clostridium perfringens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.163 4.9
-
 4-methylumbelliferyl alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranoside pH 7.4, 37°C Clostridium perfringens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.163 7.4
-
 assay at Clostridium perfringens

General Information

EC Number General Information Comment Organism
3.2.1.163 evolution the enzyme belongs to the glycoside hydrolase family 125, GH125 Streptococcus pneumoniae
3.2.1.163 evolution the enzyme belongs to the glycoside hydrolase family 125, GH125 Clostridium perfringens
3.2.1.163 physiological function the enzyme is the pneumococcal virulence factor in Streptococcus pneumoniae Streptococcus pneumoniae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.163 12.9
-
 4-methylumbelliferyl alpha-D-mannopyranosyl-(1->6)-beta-D-mannopyranoside pH 7.4, 37°C Clostridium perfringens