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Literature summary extracted from

  • Mohamed Mustafa, M.S.; Nakajima, Y.; Oyama, H.; Iwata, N.; Ito, K.
    Assessment of substrate inhibition of bacterial oligopeptidase B (2012), Biol. Pharm. Bull., 35, 2010-2016.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.83 expression in Escherichia coli Escherichia coli
3.4.21.83 expression in Escherichia coli Stenotrophomonas maltophilia
3.4.21.83 expression in Escherichia coli Rhodococcus erythropolis
3.4.21.83 expression in Escherichia coli Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.83 Escherichia coli P24555
-
-
3.4.21.83 Rhodococcus erythropolis C0ZRJ2
-
-
3.4.21.83 Serratia marcescens K0J8D4
-
-
3.4.21.83 Stenotrophomonas maltophilia K0J2A4
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.83 5
-
 substrate t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
3.4.21.83 6
-
 substrate t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
3.4.21.83 6
-
 substrate t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
3.4.21.83 6
-
 substrate t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
3.4.21.83 13
-
 substrate t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
3.4.21.83 18
-
 substrate benzoyl-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
3.4.21.83 42 60 substrate t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
3.4.21.83 53
-
 substrate benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
3.4.21.83 86
-
 substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
3.4.21.83 86
-
 substrate t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
3.4.21.83 90
-
 substrate t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
3.4.21.83 100
-
 substrate t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
3.4.21.83 105
-
 substrate benzoyl-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
3.4.21.83 124
-
 substrate t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
3.4.21.83 126
-
 substrate t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
3.4.21.83 158
-
 substrate t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
3.4.21.83 170
-
 substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
3.4.21.83 220
-
 substrate t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
3.4.21.83 243
-
 substrate t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
3.4.21.83 328
-
 substrate benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
3.4.21.83 340
-
 substrate benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
3.4.21.83 370
-
 substrate t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
3.4.21.83 382
-
 substrate t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
3.4.21.83 408
-
 substrate t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
3.4.21.83 457
-
 substrate t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3.4.21.83 519
-
 substrate t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
3.4.21.83 570
-
 substrate benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
3.4.21.83 645
-
 substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
3.4.21.83 663
-
 substrate t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
3.4.21.83 908
-
 substrate benzoyl-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
3.4.21.83 1230
-
 substrate t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
3.4.21.83 1300
-
 substrate t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3.4.21.83 1310
-
 substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3.4.21.83 1330
-
 substrate t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3.4.21.83 1430
-
 substrate benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
3.4.21.83 1810
-
 substrate benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3.4.21.83 1850
-
 substrate t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
3.4.21.83 2230
-
 substrate t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
3.4.21.83 2380
-
 substrate t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3.4.21.83 2470
-
 substrate t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3.4.21.83 3000
-
 substrate t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
3.4.21.83 3055
-
 substrate t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3.4.21.83 3130
-
 substrate benzoyl-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3.4.21.83 3275
-
 substrate t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3.4.21.83 3440
-
 substrate benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3.4.21.83 3500
-
 substrate benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
3.4.21.83 3500
-
 substrate t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3.4.21.83 3690
-
 substrate t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.83 benzoyl-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli benzoyl-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 benzoyl-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia benzoyl-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 benzoyl-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis benzoyl-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 benzoyl-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens benzoyl-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Arg-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Arg-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Arg-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Arg-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Glu-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Glu-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Glu-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Glu-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Val-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Val-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Val-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Val-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.83 20
-
-
 Escherichia coli
3.4.21.83 20
-
-
 Rhodococcus erythropolis
3.4.21.83 20
-
-
 Serratia marcescens
3.4.21.83 30
-
-
 Stenotrophomonas maltophilia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.83 30
-
 30 min, 25% loss of activity Stenotrophomonas maltophilia
3.4.21.83 40
-
 30 min, 75% loss of activiy Stenotrophomonas maltophilia
3.4.21.83 50
-
 15 min, complete loss of activity Stenotrophomonas maltophilia