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Literature summary extracted from

  • Carroll, R.K.; Veillard, F.; Gagne, D.T.; Lindenmuth, J.M.; Poreba, M.; Drag, M.; Potempa, J.; Shaw, L.N.
    The Staphylococcus aureus leucine aminopeptidase is localized to the bacterial cytosol and demonstrates a broad substrate range that extends beyond leucine (2013), Biol. Chem., 394, 791-803.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 expressed in Escherichia coli as a His-tagged fusion protein Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.79
-
 L-Arg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
3.4.11.1 0.82
-
 hArg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
3.4.11.1 1.33
-
 Phg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
3.4.11.1 1.38
-
 L-Leu-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 cytosol
-
 Staphylococcus aureus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Ca2+ enzyme not functional in the presence of Staphylococcus aureus
3.4.11.1 Cd2+ functional in the presence of, highest activity at pH 9 Staphylococcus aureus
3.4.11.1 Co2+ enzyme not functional in the presence of Staphylococcus aureus
3.4.11.1 Mg2+ enzyme not functional in the presence of Staphylococcus aureus
3.4.11.1 Mn2+ 0.2 mM, increases activity by 7fold Staphylococcus aureus
3.4.11.1 Ni2+ functional in the presence of, highest activity at pH 7 Staphylococcus aureus
3.4.11.1 Zn2+ enzyme not functional in the presence of Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 using Ni-NTA chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 hArg-7-amido-4-methylcoumarin + H2O
-
 Staphylococcus aureus hArg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Ala-7-amido-4-methylcoumarin + H2O
-
 Staphylococcus aureus L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Arg-7-amido-4-methylcoumarin + H2O
-
 Staphylococcus aureus L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-Leu-7-amido-4-methylcoumarin + H2O
-
 Staphylococcus aureus L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 additional information the substrate range of LAP is quite complex. L-Leu is not the preferred substrate of the enzyme Staphylococcus aureus ?
-
 ?
3.4.11.1 Phg-7-amido-4-methylcoumarin + H2O
-
 Staphylococcus aureus Phg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP enzyme also possesses cysteinylglycinase activity Staphylococcus aureus
3.4.11.1 leucine aminopeptidase
-
 Staphylococcus aureus
3.4.11.1 pepZ
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 37
-
 assay at Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.1 0.1204
-
 L-Leu-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
3.4.11.1 0.1395
-
 L-Arg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
3.4.11.1 0.1584
-
 hArg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
3.4.11.1 0.3952
-
 Phg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 9
-
 highest level of activity observed in TAPS or CAPS buffer. All enzymatic analyses done in TAPS buffer Staphylococcus aureus

General Information

EC Number General Information Comment Organism
3.4.11.1 malfunction inhibition of LAP with bestatin does not adversely affect growth of Staphylococcus aureus or biofilm formation Staphylococcus aureus
3.4.11.1 malfunction lysates from pepZ mutant cells show leucine aminopeptidase activity comparable to that of the wild-type strain suggesting that the majority of leucine aminopeptidase activity observed in Staphylococcus aureus cells comes from PepS, and not LAP Staphylococcus aureus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.1 0.08725
-
 L-Leu-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
3.4.11.1 0.176
-
 L-Arg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
3.4.11.1 0.193
-
 hArg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
3.4.11.1 0.297
-
 Phg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus