EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.14.10 | expressed in Escherichia coli | Drosophila melanogaster |
3.4.14.10 | expressed in Escherichia coli | Mus musculus |
3.4.14.10 | expressed in Escherichia coli | Homo sapiens |
3.4.14.10 | expression in Escherichia coli | Drosophila melanogaster |
3.4.14.10 | expression of murine TPP2 gene optimized for high-level expression in Escherichia coli | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.14.10 | D387G | mutant shows an increase in pKa for kcat | Drosophila melanogaster |
3.4.14.10 | D387G | mutant shows bell-shaped pH-dependence of kcat, possibly due to an impaired protonation of the leaving group | Mus musculus |
3.4.14.10 | D387G | the pKa values of mutant D387G for kcat (app) increase when Asp-387 is changed to glycine. Mutant shows a bell-shaped pH-dependence of kcat(app), possibly due to an impaired protonation of the leaving group. Mutant shows a decresed kcat for all substrates and a highly decreased kcat/Km compared to wild-type. In contrast to wild-type at higher pH the kcat (app) value decreases | Drosophila melanogaster |
3.4.14.10 | E331Q | mutant has a 2-3 orders of magnitude decreased kcat app/KM compared to wild-type, mostly dependent on an increase in KM | Mus musculus |
3.4.14.10 | G375D | less than 0.1% of wild-type activity | Mus musculus |
3.4.14.10 | G375D | mutant has only 0.1% activity of wild-type at pH 7.5. Mutant shows a marked decrease in pH optimum compared to wild-type | Mus musculus |
3.4.14.10 | H267A | mutant shows bell-shaped pH-dependence of kcat, possibly due to an impaired protonation of the leaving group | Mus musculus |
3.4.14.10 | H267A | mutant shows mostly a decresed kcat for all substrates and a highly decreased kcat/Km between 2 to fold orders of magnitude compared to wild-type. kcat shows a higher rise with pH in mutant H267A than in wild-type with substrate Ala-Ala-Phe-4-nitroanilide but at pH more than 7.6 kcat (app) decreases for substrate Ala-Ala-Phe-4-nitroanilide, resulting in a significantly better fit for a bell-shaped curve than for a sigmoidal | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.14.10 | 0.0036 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.008 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 0.008 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 0.009 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 0.009 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 0.01 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.01 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C | Homo sapiens | |
3.4.14.10 | 0.01 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 0.01 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 0.01 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.01 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 0.01 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 0.0106 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 7.6, 37°C | Homo sapiens | |
3.4.14.10 | 0.012 | - |
Ala-Ala-Phe-4-nitroanilide | pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.012 | - |
Ala-Ala-Phe-4-nitroanilide | pH 8.3, 37°C | Homo sapiens | |
3.4.14.10 | 0.012 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.8, 37°C | Homo sapiens | |
3.4.14.10 | 0.012 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 6.8, 37°C | Homo sapiens | |
3.4.14.10 | 0.013 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.4, 37°C | Homo sapiens | |
3.4.14.10 | 0.013 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 6.4, 37°C | Homo sapiens | |
3.4.14.10 | 0.013 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.013 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.014 | - |
Ala-Ala-Ala-4-nitroanilide | pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 0.014 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 0.015 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.015 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.017 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 0.017 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.9, 37°C | Homo sapiens | |
3.4.14.10 | 0.017 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 7.9, 37°C | Homo sapiens | |
3.4.14.10 | 0.017 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 0.024 | - |
Ala-Ala-Ala-4-nitroanilide | pH 8.3, 37°C | Homo sapiens | |
3.4.14.10 | 0.024 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 8.3, 37°C | Homo sapiens | |
3.4.14.10 | 0.024 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 0.024 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 0.028 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 0.028 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 0.03 | - |
Ala-Ala-Ala-4-nitroanilide | pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.03 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.8, 37°C | Homo sapiens | |
3.4.14.10 | 0.03 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 6.8, 37°C | Homo sapiens | |
3.4.14.10 | 0.03 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.036 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.037 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 0.037 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 0.039 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.039 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.0, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.042 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.4, 37°C | Homo sapiens | |
3.4.14.10 | 0.042 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 6.4, 37°C | Homo sapiens | |
3.4.14.10 | 0.05 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C | Homo sapiens | |
3.4.14.10 | 0.05 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 7.6, 37°C | Homo sapiens | |
3.4.14.10 | 0.05 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 0.05 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 0.05 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.05 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.2, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.05 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.7, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.05 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.7, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.054 | - |
Ala-Ala-Phe-4-nitroanilide | pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 0.054 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 0.054 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 0.054 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.0, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.056 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 0.056 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.0, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.057 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.3, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.057 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 0.058 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.058 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.06 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.9, 37°C | Homo sapiens | |
3.4.14.10 | 0.06 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 7.9, 37°C | Homo sapiens | |
3.4.14.10 | 0.06 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.06 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.06 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.2, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.06 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.3, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.062 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.062 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.062 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 0.062 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 0.07 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 0.07 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.7, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.074 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.074 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.09 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.09 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.09 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.3, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.09 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 0.1 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 0.1 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.2, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.11 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.11 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.12 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 8.3, 37°C | Homo sapiens | |
3.4.14.10 | 0.12 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.13 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.13 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.2 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.2 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.2, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.21 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.21 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.25 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.25 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.0, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.27 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.27 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.3, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.3 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.33 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.7, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.5 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 0.6 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 0.6 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 0.9 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 1.19 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 3.3 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 5 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 6 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 20 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 30 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 6.4, 37°C | Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.14.10 | Drosophila melanogaster | - |
- |
- |
3.4.14.10 | Drosophila melanogaster | Q9V6K1 | - |
- |
3.4.14.10 | Homo sapiens | - |
- |
- |
3.4.14.10 | Mus musculus | - |
- |
- |
3.4.14.10 | Mus musculus | Q64514 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.14.10 | using polyethyleneimine precipitation, (NH4)2SO4 precipitation, dialysis, anion-exchange chromatography, hydrophobic interaction chromatography | Drosophila melanogaster |
3.4.14.10 | using polyethyleneimine precipitation, (NH4)2SO4 precipitation, dialysis, anion-exchange chromatography, hydrophobic interaction chromatography | Mus musculus |
3.4.14.10 | using polyethyleneimine precipitation, (NH4)2SO4 precipitation, dialysis, anion-exchange chromatography, hydrophobic interaction chromatography | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.14.10 | commercial preparation | - |
Homo sapiens | - |
3.4.14.10 | erythrocyte | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.14.10 | Ala-Ala-Ala-4-nitroanilide + H2O | - |
Drosophila melanogaster | Ala-Ala-Ala + 4-nitroaniline | - |
? | |
3.4.14.10 | Ala-Ala-Ala-4-nitroanilide + H2O | - |
Mus musculus | Ala-Ala-Ala + 4-nitroaniline | - |
? | |
3.4.14.10 | Ala-Ala-Ala-4-nitroanilide + H2O | - |
Homo sapiens | Ala-Ala-Ala + 4-nitroaniline | - |
? | |
3.4.14.10 | Ala-Ala-Phe-4-nitroanilide + H2O | - |
Drosophila melanogaster | Ala-Ala-Phe + 4-nitroaniline | - |
? | |
3.4.14.10 | Ala-Ala-Phe-4-nitroanilide + H2O | - |
Mus musculus | Ala-Ala-Phe + 4-nitroaniline | - |
? | |
3.4.14.10 | Ala-Ala-Phe-4-nitroanilide + H2O | - |
Homo sapiens | Ala-Ala-Phe + 4-nitroaniline | - |
? | |
3.4.14.10 | L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O | both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme | Homo sapiens | L-Ala-L-Ala-L-Ala + 4-nitroaniline | - |
? | |
3.4.14.10 | L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O | both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme | Mus musculus | L-Ala-L-Ala-L-Ala + 4-nitroaniline | - |
? | |
3.4.14.10 | L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O | both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme | Drosophila melanogaster | L-Ala-L-Ala-L-Ala + 4-nitroaniline | - |
? | |
3.4.14.10 | L-Ala-L-Ala-L-Phe 4-nitroanilide + H2O | both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme | Homo sapiens | L-Ala-L-Ala-L-Phe + 4-nitroaniline | - |
? | |
3.4.14.10 | L-Ala-L-Ala-L-Phe 4-nitroanilide + H2O | both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme | Mus musculus | L-Ala-L-Ala-L-Phe + 4-nitroaniline | - |
? | |
3.4.14.10 | L-Ala-L-Ala-L-Phe 4-nitroanilide + H2O | both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme | Drosophila melanogaster | L-Ala-L-Ala-L-Phe + 4-nitroaniline | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.14.10 | ? | x * 100000-140000, SDS-PAGE of recombinant protein, full-length TPP II and shortened versions thereof | Mus musculus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.14.10 | dTPP II | - |
Drosophila melanogaster |
3.4.14.10 | hTPP II | - |
Homo sapiens |
3.4.14.10 | mTPP II | - |
Mus musculus |
3.4.14.10 | tripeptidyl aminopeptidase II | - |
Drosophila melanogaster |
3.4.14.10 | tripeptidyl aminopeptidase II | - |
Mus musculus |
3.4.14.10 | tripeptidyl aminopeptidase II | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.14.10 | 37 | - |
assay at | Drosophila melanogaster |
3.4.14.10 | 37 | - |
assay at | Mus musculus |
3.4.14.10 | 37 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.14.10 | 0.25 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 0.25 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.2, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.38 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 0.38 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.2, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.441 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 0.441 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.509 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 0.509 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.7, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.58 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 0.58 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.0, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.74 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.74 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.857 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 0.857 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.86 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 0.86 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.3, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.9 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 0.9 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 0.9 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.7, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.9 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.0, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 1 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 1 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 1.12 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 1.12 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.3, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 1.29 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 1.29 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.3, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 1.3 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 1.3 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.2, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 1.42 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 1.42 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.0, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 1.5 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 1.5 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.7, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 2 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.4, 37°C | Homo sapiens | |
3.4.14.10 | 2 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 6.4, 37°C | Homo sapiens | |
3.4.14.10 | 3 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 3 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.3, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 3.1 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C | Homo sapiens | |
3.4.14.10 | 3.1 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 7.6, 37°C | Homo sapiens | |
3.4.14.10 | 3.3 | - |
Ala-Ala-Ala-4-nitroanilide | pH 8.3, 37°C | Homo sapiens | |
3.4.14.10 | 3.3 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 8.3, 37°C | Homo sapiens | |
3.4.14.10 | 4 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.8, 37°C | Homo sapiens | |
3.4.14.10 | 4 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 6.8, 37°C | Homo sapiens | |
3.4.14.10 | 4 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 4 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 4.2 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 5 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.9, 37°C | Homo sapiens | |
3.4.14.10 | 5 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 7.9, 37°C | Homo sapiens | |
3.4.14.10 | 5 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 5 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.2, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 6 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 6 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.0, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 7.2 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 7.2 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.7, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 9.6 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.4, 37°C | Homo sapiens | |
3.4.14.10 | 9.6 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 6.4, 37°C | Homo sapiens | |
3.4.14.10 | 10 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.8, 37°C | Homo sapiens | |
3.4.14.10 | 10 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 6.8, 37°C | Homo sapiens | |
3.4.14.10 | 10 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 10 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 11 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 11 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 11 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 12 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 12 | - |
Ala-Ala-Ala-4-nitroanilide | pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 12 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 12 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 13 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 13 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 13 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 13 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 18 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 19 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.9, 37°C | Homo sapiens | |
3.4.14.10 | 19 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 7.9, 37°C | Homo sapiens | |
3.4.14.10 | 21 | - |
Ala-Ala-Phe-4-nitroanilide | pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 21 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 21 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 22 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C | Homo sapiens | |
3.4.14.10 | 22 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 7.6, 37°C | Homo sapiens | |
3.4.14.10 | 27 | - |
Ala-Ala-Phe-4-nitroanilide | pH 8.3, 37°C | Homo sapiens | |
3.4.14.10 | 27 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 8.3, 37°C | Homo sapiens | |
3.4.14.10 | 27 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 27 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 30 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 30 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 33 | - |
Ala-Ala-Ala-4-nitroanilide | pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 33 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 33 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 33 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 38 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 38 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 42 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 42 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 50 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 50 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 56.8 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 57 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 57 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 58.8 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 60 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 60 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 65 | - |
Ala-Ala-Phe-4-nitroanilide | pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 65 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 80 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 96 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 96 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 100 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 114 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 114 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 125 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 500 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 6.4, 37°C | Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.14.10 | 7.5 | - |
assay at | Drosophila melanogaster |
3.4.14.10 | 7.5 | - |
assay at | Mus musculus |
3.4.14.10 | 7.5 | - |
assay at | Homo sapiens |
3.4.14.10 | 7.6 | - |
optimal pH, substrate: Ala-Ala-Phe-4-nitroanilide | Drosophila melanogaster |
3.4.14.10 | 7.9 | - |
optimal pH, substrate: Ala-Ala-Phe-4-nitroanilide | Mus musculus |
3.4.14.10 | 7.9 | - |
optimal pH, substrate: Ala-Ala-Phe-4-nitroanilide | Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.14.10 | additional information | - |
isoform TPP II has a bell-shaped pH dependence of kcat app/KM probably due to deprotonation of the N-terminal amino group of the substrate at higher pH | Homo sapiens |
3.4.14.10 | additional information | - |
isoform TPP II has a bell-shaped pH dependence of kcat app/KM probably due to deprotonation of the N-terminal amino group of the substrate at higher pH | Mus musculus |
3.4.14.10 | additional information | - |
isoform TPP II has a bell-shaped pH dependence of kcat app/KM probably due to deprotonation of the N-terminal amino group of the substrate at higher pH | Drosophila melanogaster |
3.4.14.10 | additional information | - |
kcat(app) increases with pH | Drosophila melanogaster |
3.4.14.10 | additional information | - |
kcat(app) increases with pH | Mus musculus |
3.4.14.10 | additional information | - |
kcat(app) increases with pH | Homo sapiens |
3.4.14.10 | 7 | 8 | optimal pH, substrate: Ala-Ala-Ala-4-nitroanilide | Drosophila melanogaster |
3.4.14.10 | 7 | 8 | optimal pH, substrate: Ala-Ala-Ala-4-nitroanilide | Mus musculus |
3.4.14.10 | 7 | 8 | optimal pH, substrate: Ala-Ala-Ala-4-nitroanilide | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.14.10 | 0.0000038 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.2, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.000005 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.2, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.000006 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.000006 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.000007 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.00001 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.7, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.00001 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.3, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.000011 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.0, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.000012 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.3, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.000013 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.7, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.000014 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.0000165 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.0, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.00002 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.3, 37°C, mutant H267A | Mus musculus | |
3.4.14.10 | 0.000021 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.2, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.000021 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.3, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.000022 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.7, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.000023 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.0, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.00003 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.2, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.00003 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.7, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.000036 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.0, 37°C, mutant D387G | Drosophila melanogaster | |
3.4.14.10 | 0.000138 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.4, 37°C | Homo sapiens | |
3.4.14.10 | 0.00016 | - |
Ala-Ala-Ala-4-nitroanilide | pH 8.3, 37°C | Homo sapiens | |
3.4.14.10 | 0.00019 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.00023 | - |
Ala-Ala-Ala-4-nitroanilide | pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 0.00023 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.4, 37°C | Homo sapiens | |
3.4.14.10 | 0.00025 | - |
Ala-Ala-Phe-4-nitroanilide | pH 8.3, 37°C | Homo sapiens | |
3.4.14.10 | 0.00028 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C | Homo sapiens | |
3.4.14.10 | 0.0003 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.9, 37°C | Homo sapiens | |
3.4.14.10 | 0.0003 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.8, 37°C | Homo sapiens | |
3.4.14.10 | 0.00031 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.8, 37°C | Homo sapiens | |
3.4.14.10 | 0.00033 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.9, 37°C | Homo sapiens | |
3.4.14.10 | 0.00033 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.00037 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C | Homo sapiens | |
3.4.14.10 | 0.0005 | - |
Ala-Ala-Ala-4-nitroanilide | pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.00056 | - |
Ala-Ala-Phe-4-nitroanilide | pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.00056 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.00082 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.0009 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.00098 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.001 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.0015 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 0.0018 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 0.0025 | - |
Ala-Ala-Phe-4-nitroanilide | pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 0.0026 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 0.0028 | - |
Ala-Ala-Phe-4-nitroanilide | pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 0.0029 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 0.0031 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 0.0035 | - |
Ala-Ala-Phe-4-nitroanilide | pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 0.0038 | - |
Ala-Ala-Ala-4-nitroanilide | pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 0.0039 | - |
Ala-Ala-Ala-4-nitroanilide | pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 3.4 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 3.82 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 5 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 6 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 6 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 6 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 6.7 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 7.1 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 8 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 9 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 9.6 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant E331Q, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 10 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 10 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 11 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 12 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 13 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 14 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 15 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 15 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 16.5 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant H267A, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 17 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 20 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant E331Q, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 20 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant H267A, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 21 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 21 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 22 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 23 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 30 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | mutant D387G, pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 30 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 36 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | mutant D387G, pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 138 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 6.4, 37°C | Homo sapiens | |
3.4.14.10 | 160 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 8.3, 37°C | Homo sapiens | |
3.4.14.10 | 190 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 230 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 6.4, 37°C | Homo sapiens | |
3.4.14.10 | 250 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 8.3, 37°C | Homo sapiens | |
3.4.14.10 | 280 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 7.6, 37°C | Homo sapiens | |
3.4.14.10 | 300 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 7.9, 37°C | Homo sapiens | |
3.4.14.10 | 300 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | pH 6.8, 37°C | Homo sapiens | |
3.4.14.10 | 310 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 6.8, 37°C | Homo sapiens | |
3.4.14.10 | 330 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 7.9, 37°C | Homo sapiens | |
3.4.14.10 | 330 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 6.4, 37°C | Drosophila melanogaster | |
3.4.14.10 | 370 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | pH 7.6, 37°C | Homo sapiens | |
3.4.14.10 | 500 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 560 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 560 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 8.3, 37°C | Drosophila melanogaster | |
3.4.14.10 | 820 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 900 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 6.8, 37°C | Drosophila melanogaster | |
3.4.14.10 | 980 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 1000 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 7.9, 37°C | Drosophila melanogaster | |
3.4.14.10 | 1500 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 7.6, 37°C | Drosophila melanogaster | |
3.4.14.10 | 1800 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 2100 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 6.4, 37°C | Mus musculus | |
3.4.14.10 | 2500 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 2600 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 8.3, 37°C | Mus musculus | |
3.4.14.10 | 2800 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 2900 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 6.8, 37°C | Mus musculus | |
3.4.14.10 | 3100 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 3500 | - |
L-Ala-L-Ala-L-Phe 4-nitroanilide | wild-type, pH 7.6, 37°C | Mus musculus | |
3.4.14.10 | 3800 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 7.9, 37°C | Mus musculus | |
3.4.14.10 | 3900 | - |
L-Ala-L-Ala-L-Ala 4-nitroanilide | wild-type, pH 7.6, 37°C | Mus musculus |