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Literature summary extracted from

  • Damasio, A.R.; Ribeiro, L.F.; Ribeiro, L.F.; Furtado, G.P.; Segato, F.; Almeida, F.B.; Crivellari, A.C.; Buckeridge, M.S.; Souza, T.A.; Murakami, M.T.; Ward, R.J.; Prade, R.A.; Polizeli, M.L.
    Functional characterization and oligomerization of a recombinant xyloglucan-specific endo-beta-1,4-glucanase (GH12) from Aspergillus niveus (2012), Biochim. Biophys. Acta, 1824, 461-467.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.151 by gel filtration Aspergillus niveus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 additional information
-
 additional information kinetics with tamarind xyloglucan Aspergillus niveus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.151 cell wall
-
 Aspergillus niveus 5618
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.151 23500
-
 x * 23500, about, sequence calculation, the enzyme is a predominantly a monomer at 60°C and is more active in its monomeric state Aspergillus niveus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.151 Aspergillus niveus
-
 gene xegA
-
3.2.1.151 Aspergillus niveus A773
-
 gene xegA
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.151 mycelium
-
 Aspergillus niveus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.151 tamarind xyloglucan + H2O
-
 Aspergillus niveus ?
-
 ?
3.2.1.151 tamarind xyloglucan + H2O
-
 Aspergillus niveus A773 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.151 monomer or dimer x * 23500, about, sequence calculation, the enzyme is a predominantly a monomer at 60°C and is more active in its monomeric state Aspergillus niveus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.151 GH12 xyloglucanase
-
 Aspergillus niveus
3.2.1.151 XegA
-
 Aspergillus niveus
3.2.1.151 xyloglucan-specific beta-(1,4)-glucanase
-
 Aspergillus niveus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.151 60
-
-
 Aspergillus niveus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.151 20 80 activity range Aspergillus niveus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.151 60
-
 thermal unfolding studies displays two transitions with mid-point temperatures of 51.3°C and 81.3°C respectively, and dynamic light scattering studies indicate that the first transition involves a change in oligomeric state from a dimeric to a monomeric form Aspergillus niveus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 6
-
-
 Aspergillus niveus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.151 4 7.5 activity range Aspergillus niveus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.151 Aspergillus niveus sequence calculation
-
 4.38

General Information

EC Number General Information Comment Organism
3.2.1.151 evolution the enzyme belongs to the glucohydrolase family 12, GH12 Aspergillus niveus
3.2.1.151 additional information structure homology modelling using Aspergillus niger endoglucanase structure, PDB ID 1KS5 Aspergillus niveus