EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.11.48 | overproduction of His6-tagged enzyme in Aspergillus nidulans and Escherichia coli | Aspergillus nidulans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.48 | 6,8-dihydroxypurine | 0.08 mM, 24% decrease in activity | Aspergillus nidulans | |
1.14.11.48 | Co2+ | 0.04 mM, about 65% inhibition | Aspergillus nidulans | |
1.14.11.48 | Cu2+ | 0.04 mM, complete inhibition | Aspergillus nidulans | |
1.14.11.48 | Fe2+ | depending on the experimental conditions, Fe2+ concentrations of above 0.025 mM can lead to enzyme inhibition of the recombinant enzyme isolated from Escherichia coli, whereas the enzyme from Aspergillus nidulans is not inhibited at Fe2+ concentrations up to 0.080 mM | Aspergillus nidulans | |
1.14.11.48 | Mn2+ | 0.04 mM, about 80% inhibition | Aspergillus nidulans | |
1.14.11.48 | N-oxalylglycine | competes with 2-oxoglutarate | Aspergillus nidulans | |
1.14.11.48 | NaCl | 0.5 M NaCl increases the Km of 2-oxoglutarate, increases the Km of xanthine and decreased the kcat | Aspergillus nidulans | |
1.14.11.48 | Zn2+ | 0.04 mM, complete inhibition | Aspergillus nidulans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.11.48 | 0.0311 | - |
2-oxoglutarate | 25°C, pH 7.4, recombinant enzyme isolated from Escherichia coli | Aspergillus nidulans | |
1.14.11.48 | 0.0452 | - |
xanthine | 25°C, pH 7.4, recombinant enzyme isolated from Escherichia coli | Aspergillus nidulans | |
1.14.11.48 | 0.046 | - |
xanthine | 30°C, pH 7.4, enzyme isolated from Aspergillus nidulans | Aspergillus nidulans | |
1.14.11.48 | 0.05 | - |
2-oxoglutarate | 30°C, pH 7.4, enzyme isolated from Aspergillus nidulans | Aspergillus nidulans | |
1.14.11.48 | 0.16 | - |
2-oxoadipate | 25°C, pH 7.4, recombinant enzyme isolated from Escherichia coli | Aspergillus nidulans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.48 | Fe2+ | reqired. Half-maximal activity at 0.007 mM when the recombinant apoprotein isolated from the Escherichia coli is used | Aspergillus nidulans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.11.48 | 40000 | - |
12 * 40000, SDS-PAGE | Aspergillus nidulans |
1.14.11.48 | 500000 | - |
dodecamer, gel filtration | Aspergillus nidulans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.48 | Aspergillus nidulans | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.14.11.48 | glycoprotein | presence of O-glycosylation as well as N-glycosylation | Aspergillus nidulans |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.11.48 | purified from both the fungal mycelium and recombinant Escherichia coli cells | Aspergillus nidulans |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.11.48 | mycelium | - |
Aspergillus nidulans | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.11.48 | -80°C, stable for at least 2 months | Aspergillus nidulans |
1.14.11.48 | 4°C, stored in 100 mM Tris buffer (pH 7) containing 300 mM NaCl, 250 mM imidazole, 15% glycerol, and 0.005 mM Fe2+, concentrated enzyme is stable for at least 5 months | Aspergillus nidulans |
1.14.11.48 | 4°C, stored in 100 mM Tris buffer (pH 8.0) containing 300 mM NaCl, 250 mM imidazole, 1 mM EDTA, and 15% glycerol, stable for at least 1 month | Aspergillus nidulans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.48 | additional information | no activity with pyruvate, 2-oxobutyrate, phenyl pyruvate, and 4-hydroxyphenyl pyruvate | Aspergillus nidulans | ? | - |
? | |
1.14.11.48 | xanthine + 2-oxoadipate + O2 | - |
Aspergillus nidulans | ? | - |
? | |
1.14.11.48 | xanthine + 2-oxoglutarate + O2 | the enzyme is highly specific for xanthine. On the basis of the spectroscopic assay using standard conditions with 12 nM enzyme, no activity is detected when the enzyme is assayed with 0.08, 0.1, or 0.2 mM hypoxanthine, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, 9-methylxanthine, purine, 6-methylpurine, 2-hydroxypurine, 8-hydroxypurine, 2,8-dihydroxypurine, 2-hydroxy-6-methylpurine, allopurinol, allantoin, or adenosine diphosphate | Aspergillus nidulans | urate + succinate + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.11.48 | dodecamer | 12 * 40000, SDS-PAGE | Aspergillus nidulans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.11.48 | XanA | - |
Aspergillus nidulans |
1.14.11.48 | xanthine/alpha-ketoglutarate dioxygenase | - |
Aspergillus nidulans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.11.48 | 7.6 | - |
2-oxoadipate | 25°C, pH 7.4, recombinant enzyme isolated from Escherichia coli | Aspergillus nidulans | |
1.14.11.48 | 15 | 30 | 2-oxoglutarate | 30°C, pH 7.4, value depending on preparation, enzyme isolated from Aspergillus nidulans | Aspergillus nidulans | |
1.14.11.48 | 15 | 30 | xanthine | 30°C, pH 7.4, value depending on preparation, enzyme isolated from Aspergillus nidulans | Aspergillus nidulans | |
1.14.11.48 | 66.5 | - |
2-oxoglutarate | 25°C, pH 7.4, recombinant enzyme isolated from Escherichia coli | Aspergillus nidulans | |
1.14.11.48 | 71.4 | - |
xanthine | 25°C, pH 7.4, recombinant enzyme isolated from Escherichia coli | Aspergillus nidulans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.11.48 | 7 | 8 | the recombinant enzyme isolated from Escherichia coli exhibits a narrow pH optimum of 7.0-8.0 with pH 7.4 yielding optimal activity for Tris, MOPS, MES, imidazole, and HEPES buffers | Aspergillus nidulans |
1.14.11.48 | 7 | - |
enzyme isolated from Aspergillus nidulans | Aspergillus nidulans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.11.48 | 7 | 11 | stable | Aspergillus nidulans |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.11.48 | 0.00012 | - |
N-oxalylglycine | pH 7.4, 25°C | Aspergillus nidulans |