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Literature summary extracted from

  • Sinha, S.; Chand, S.; Tripathi, P.
    Production, purification and characterization of a new chitosanase enzyme and improvement of chitosan pentamer and hexamer yield in an enzyme membrane reactor (2014), Biocatal. Biotransform., 32, 208-213.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.132 extracellular
-
 Streptomyces zaomyceticus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 95000
-
 gel filtration Streptomyces zaomyceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Streptomyces zaomyceticus
-
 chitosan oligosaccharides
-
 ?
3.2.1.132 chitosan + H2O Streptomyces zaomyceticus C6
-
 chitosan oligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Streptomyces zaomyceticus
-
-
-
3.2.1.132 Streptomyces zaomyceticus C6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 acetone fractionation and column chromatography Streptomyces zaomyceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Streptomyces zaomyceticus chitosan oligosaccharides
-
 ?
3.2.1.132 chitosan + H2O
-
 Streptomyces zaomyceticus C6 chitosan oligosaccharides
-
 ?
3.2.1.132 additional information the enzyme is not able to degrade chitin Streptomyces zaomyceticus ?
-
 ?
3.2.1.132 additional information the enzyme is not able to degrade chitin Streptomyces zaomyceticus C6 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 monomer 1 * 95000, SDS-PAGE Streptomyces zaomyceticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 60
-
 at pH 5.5 Streptomyces zaomyceticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132
-
 90 the enzyme is stable over a temperature range of 0-50°C and shows about 20% of the initial activity after heating at 90°C for 10 min Streptomyces zaomyceticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 4.5 6.5 the enzyme is stable over a a pH range of 4.5-6.5 Streptomyces zaomyceticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.132 Streptomyces zaomyceticus isoelectric focusing
-
 6.5