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Literature summary extracted from

  • Kamita, S.G.; Oshita, G.H.; Wang, P.; Morisseau, C.; Hammock, B.D.; Nandety, R.S.; Falk, B.W.
    Characterization of Hovi-mEH1, a microsomal epoxide hydrolase from the glassy-winged sharpshooter Homalodisca vitripennis (2013), Arch. Insect Biochem. Physiol., 83, 171-179.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.3.2.9 expressed in Sf-9 insect cells Homalodisca vitripennis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.9 0.0138
-
 juvenile hormone III at pH 8.0 and 30°C Homalodisca vitripennis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.3.2.9 microsome
-
 Homalodisca vitripennis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.3.2.9 52100
-
 x * 52100, SDS-PAGE Homalodisca vitripennis
3.3.2.9 52108
-
 x * 52108, calculated from amino acid sequence Homalodisca vitripennis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.3.2.9 cis-stilbene oxide + H2O Homalodisca vitripennis
-
 (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.9 Homalodisca vitripennis S4UPN4 glassy-winged sharpshooter
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3.2.9 0.00036
-
 with 14,15-epoxyeicosatrienoic acid as substrate, at pH 8.0 and 30°C Homalodisca vitripennis
3.3.2.9 0.0013
-
 with trans-diphenylpropene oxide as substrate, at pH 7.0 and 30°C Homalodisca vitripennis
3.3.2.9 0.0293
-
 with juvenile hormone III as substrate, at pH 8.0 and 30°C Homalodisca vitripennis
3.3.2.9 0.0475
-
 with cis-stilbene oxide as substrate, at pH 7.0 and 30°C Homalodisca vitripennis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.9 14,15-epoxyeicosatrienoic acid + H2O
-
 Homalodisca vitripennis ?
-
 ?
3.3.2.9 cis-stilbene oxide + H2O
-
 Homalodisca vitripennis (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
3.3.2.9 juvenile hormone III + H2O
-
 Homalodisca vitripennis ?
-
 ?
3.3.2.9 additional information no activity with trans-stilbene oxide Homalodisca vitripennis ?
-
 ?
3.3.2.9 trans-diphenylpropene oxide + H2O
-
 Homalodisca vitripennis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.3.2.9 ? x * 52100, SDS-PAGE Homalodisca vitripennis
3.3.2.9 ? x * 52108, calculated from amino acid sequence Homalodisca vitripennis

Synonyms

EC Number Synonyms Comment Organism
3.3.2.9 mEH1
-
 Homalodisca vitripennis
3.3.2.9 xenobiotic epoxide hydrolase
-
 Homalodisca vitripennis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3.2.9 0.03
-
 juvenile hormone III at pH 8.0 and 30°C Homalodisca vitripennis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.3.2.9 Homalodisca vitripennis calculated from amino acid sequence
-
 7