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Literature summary extracted from

  • Sakurama, H.; Kishino, S.; Uchibori, Y.; Yonejima, Y.; Ashida, H.; Kita, K.; Takahashi, S.; Ogawa, J.
    beta-Glucuronidase from Lactobacillus brevis useful for baicalin hydrolysis belongs to glycoside hydrolase family 30 (2014), Appl. Microbiol. Biotechnol., 98, 4021-4032.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.167 DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) Levilactobacillus brevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.167 0.154
-
4-nitrophenyl beta-D-glucuronide native enzyme, pH 5.0, 37°C Levilactobacillus brevis
3.2.1.167 0.156
-
4-nitrophenyl beta-D-glucuronide recombinant His-tagged enzyme, pH 5.0, 37°C Levilactobacillus brevis
3.2.1.167 1.5 2 Baicalin native enzyme, pH 5.0, 37°C Levilactobacillus brevis
3.2.1.167 1.74
-
Baicalin recombinant His-tagged enzyme, pH 5.0, 37°C Levilactobacillus brevis
3.2.1.167 2.02
-
wogonoside recombinant His-tagged enzyme, pH 5.0, 37°C Levilactobacillus brevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.167 60000
-
x * 60000, native enzyme, SDS-PAGE Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.167 baicalin + H2O Levilactobacillus brevis the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones baicalein + D-glucuronate
-
?
3.2.1.167 baicalin + H2O Levilactobacillus brevis FERM BP-4693 the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones baicalein + D-glucuronate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.167 Levilactobacillus brevis
-
subsp. coagulans
-
3.2.1.167 Levilactobacillus brevis FERM BP-4693
-
subsp. coagulans
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.167 recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography and dialysis, native enzyme 1800fold from cell-free extract by repeated anion exchange chromatography and gel filtration, followed by dialysis Levilactobacillus brevis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.167 7.57
-
purified native enzyme, pH 5.0, 37°C, substrate baicalin Levilactobacillus brevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.167 4-nitrophenyl beta-D-glucuronide + H2O
-
Levilactobacillus brevis 4-nitrophenol + D-glucuronate
-
?
3.2.1.167 4-nitrophenyl beta-D-glucuronide + H2O
-
Levilactobacillus brevis FERM BP-4693 4-nitrophenol + D-glucuronate
-
?
3.2.1.167 baicalin + H2O the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones Levilactobacillus brevis baicalein + D-glucuronate
-
?
3.2.1.167 baicalin + H2O the enzyme is strictly glycon-specific but not aglycon-specific Levilactobacillus brevis baicalein + D-glucuronate
-
?
3.2.1.167 baicalin + H2O the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones Levilactobacillus brevis FERM BP-4693 baicalein + D-glucuronate
-
?
3.2.1.167 baicalin + H2O the enzyme is strictly glycon-specific but not aglycon-specific Levilactobacillus brevis FERM BP-4693 baicalein + D-glucuronate
-
?
3.2.1.167 estrone 3-(beta-D-glucuronide) + H2O
-
Levilactobacillus brevis estrone + D-glucuronate
-
?
3.2.1.167 estrone 3-(beta-D-glucuronide) + H2O
-
Levilactobacillus brevis FERM BP-4693 estrone + D-glucuronate
-
?
3.2.1.167 additional information substrate specificity, the enzyme is inactive with other 4-nitrophenyl glycosides, overview Levilactobacillus brevis ?
-
?
3.2.1.167 additional information substrate specificity, the enzyme is inactive with other 4-nitrophenyl glycosides, overview Levilactobacillus brevis FERM BP-4693 ?
-
?
3.2.1.167 wogonoside + H2O
-
Levilactobacillus brevis ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.167 ? x * 60000, native enzyme, SDS-PAGE Levilactobacillus brevis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.167 LcGUS30
-
Levilactobacillus brevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.167 37
-
assay at Levilactobacillus brevis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.167 20 80 activity range, profile overview Levilactobacillus brevis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.167 20 60 purified enzyme, stable at Levilactobacillus brevis
3.2.1.167 70
-
purified enzyme, inactivation Levilactobacillus brevis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.167 6.37
-
wogonoside recombinant His-tagged enzyme, pH 5.0, 37°C Levilactobacillus brevis
3.2.1.167 18.5
-
Baicalin recombinant His-tagged enzyme, pH 5.0, 37°C Levilactobacillus brevis
3.2.1.167 21.3
-
Baicalin native enzyme, pH 5.0, 37°C Levilactobacillus brevis
3.2.1.167 31.8
-
4-nitrophenyl beta-D-glucuronide recombinant His-tagged enzyme, pH 5.0, 37°C Levilactobacillus brevis
3.2.1.167 53.7
-
4-nitrophenyl beta-D-glucuronide native enzyme, pH 5.0, 37°C Levilactobacillus brevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.167 5
-
assay at Levilactobacillus brevis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.167 3.5 7 activity range, profile overview Levilactobacillus brevis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.167 3
-
purified enzyme, inactivation Levilactobacillus brevis
3.2.1.167 3.5 9 purified enzyme, stable at Levilactobacillus brevis

General Information

EC Number General Information Comment Organism
3.2.1.167 evolution the enzyme belongs to the glycoside hydrolase family 30, GH30, subfamily 3 Levilactobacillus brevis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.167 2.58
-
estrone 3-(beta-D-glucuronide) recombinant His-tagged enzyme, pH 5.0, 37°C Levilactobacillus brevis
3.2.1.167 3.15
-
wogonoside recombinant His-tagged enzyme, pH 5.0, 37°C Levilactobacillus brevis
3.2.1.167 10.6
-
Baicalin recombinant His-tagged enzyme, pH 5.0, 37°C Levilactobacillus brevis
3.2.1.167 14
-
Baicalin native enzyme, pH 5.0, 37°C Levilactobacillus brevis
3.2.1.167 204
-
4-nitrophenyl beta-D-glucuronide recombinant His-tagged enzyme, pH 5.0, 37°C Levilactobacillus brevis
3.2.1.167 348
-
4-nitrophenyl beta-D-glucuronide native enzyme, pH 5.0, 37°C Levilactobacillus brevis