EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.5.2 | expression in Escherichia coli | Rhizorhabdus wittichii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.5.2 | CuSO4 | 10 mM, almost complete inhibition | Rhizorhabdus wittichii | |
1.2.5.2 | EDTA | 1 mM, 25% decrease in activity | Rhizorhabdus wittichii | |
1.2.5.2 | FeCl3 | 10 mM, almost complete inhibition | Rhizorhabdus wittichii | |
1.2.5.2 | ZnSO4 | 10 mM, almost complete inhibition | Rhizorhabdus wittichii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.5.2 | 12.3 | - |
Butanal | pH 8, 25°C | Rhizorhabdus wittichii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.2.5.2 | periplasm | - |
Rhizorhabdus wittichii | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.5.2 | 39300 | - |
12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus some octamer | Rhizorhabdus wittichii |
1.2.5.2 | 39300 | - |
minor protein band, 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus dodecamer | Rhizorhabdus wittichii |
1.2.5.2 | 39329 | - |
12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus some octamer | Rhizorhabdus wittichii |
1.2.5.2 | 39329 | - |
minor protein band, 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus dodecamer | Rhizorhabdus wittichii |
1.2.5.2 | 39400 | - |
12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus some octamer | Rhizorhabdus wittichii |
1.2.5.2 | 39400 | - |
minor protein band, 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus dodecamer | Rhizorhabdus wittichii |
1.2.5.2 | 330000 | - |
PAGE, minor protein band | Rhizorhabdus wittichii |
1.2.5.2 | 450000 | - |
PAGE | Rhizorhabdus wittichii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.5.2 | Rhizorhabdus wittichii | A5VEL7 | - |
- |
1.2.5.2 | Rhizorhabdus wittichii DSM 6014 | A5VEL7 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.5.2 | 180 | - |
substrate L-sorbosone, pH 8, 25°C | Rhizorhabdus wittichii |
1.2.5.2 | 1050 | - |
substrate glyoxal, pH 8, 25°C | Rhizorhabdus wittichii |
1.2.5.2 | 1060 | - |
substrate glycolaldehyde, pH 8, 25°C | Rhizorhabdus wittichii |
1.2.5.2 | 1170 | - |
substrate 3-methylbenzaldehyde, pH 8, 25°C | Rhizorhabdus wittichii |
1.2.5.2 | 1450 | - |
substrate valeraldehyde, pH 8, 25°C | Rhizorhabdus wittichii |
1.2.5.2 | 1690 | - |
substrate methylglyoxal, pH 8, 25°C | Rhizorhabdus wittichii |
1.2.5.2 | 1740 | - |
substrate propanal, pH 8, 25°C | Rhizorhabdus wittichii |
1.2.5.2 | 1900 | - |
substrate phenylglyoxal, pH 8, 25°C | Rhizorhabdus wittichii |
1.2.5.2 | 2470 | - |
substrate butanal, pH 8, 25°C | Rhizorhabdus wittichii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.5.2 | 3-methylbenzaldehyde + ferricyanide | - |
Rhizorhabdus wittichii | 3-methylbenzoic acid + ferrocyanide + H+ | - |
? | |
1.2.5.2 | 3-methylbenzaldehyde + ferricyanide | - |
Rhizorhabdus wittichii DSM 6014 | 3-methylbenzoic acid + ferrocyanide + H+ | - |
? | |
1.2.5.2 | butanal + ferricyanide | - |
Rhizorhabdus wittichii | butanoic acid + ferrocyanide + H+ | - |
? | |
1.2.5.2 | butanal + ferricyanide | - |
Rhizorhabdus wittichii DSM 6014 | butanoic acid + ferrocyanide + H+ | - |
? | |
1.2.5.2 | glycolaldehyde + ferricyanide | - |
Rhizorhabdus wittichii | hydroxyacetic acid + ferrocyanide + H+ | - |
? | |
1.2.5.2 | glycolaldehyde + ferricyanide | - |
Rhizorhabdus wittichii DSM 6014 | hydroxyacetic acid + ferrocyanide + H+ | - |
? | |
1.2.5.2 | glyoxal + ferricyanide | - |
Rhizorhabdus wittichii | oxoacetic acid + ferrocyanide + H+ | - |
? | |
1.2.5.2 | glyoxal + ferricyanide | - |
Rhizorhabdus wittichii DSM 6014 | oxoacetic acid + ferrocyanide + H+ | - |
? | |
1.2.5.2 | L-sorbosone + ferricyanide | - |
Rhizorhabdus wittichii | ? + ferrocyanide + H+ | - |
? | |
1.2.5.2 | methylglyoxal + ferricyanide | - |
Rhizorhabdus wittichii | 2-oxopropanoic acid + ferrocyanide + H+ | - |
? | |
1.2.5.2 | additional information | the enzyme can also transfer electrons to the electron acceptor system DCPIP/PMS, but the activity is only 50% of the rate obtained with ferricyanide. Enzyme displays a broad substrate spectrum and oxidizes short- and medium-chain aliphatic aldehydes (C1-C6), ketoaldehydes, dialdehydes; some aromatic aldehydes and hydroxyaldehydes to the corresponding carboxylic acids | Rhizorhabdus wittichii | ? | - |
? | |
1.2.5.2 | additional information | the enzyme can also transfer electrons to the electron acceptor system DCPIP/PMS, but the activity is only 50% of the rate obtained with ferricyanide. Enzyme displays a broad substrate spectrum and oxidizes short- and medium-chain aliphatic aldehydes (C1-C6), ketoaldehydes, dialdehydes; some aromatic aldehydes and hydroxyaldehydes to the corresponding carboxylic acids | Rhizorhabdus wittichii DSM 6014 | ? | - |
? | |
1.2.5.2 | phenylglyoxal + ferricyanide | - |
Rhizorhabdus wittichii | oxo(phenyl) acetic acid + ferrocyanide + H+ | - |
? | |
1.2.5.2 | propanal + ferricyanide | - |
Rhizorhabdus wittichii | propanoic acid + ferrocyanide + H+ | - |
? | |
1.2.5.2 | valeraldehyde + ferricyanide | - |
Rhizorhabdus wittichii | pentanoic acid + ferrocyanide + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.5.2 | dodecamer | 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus some octamer | Rhizorhabdus wittichii |
1.2.5.2 | octamer | minor protein band, 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus dodecamer | Rhizorhabdus wittichii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.5.2 | glucose sorbosone dehydrogenase | - |
Rhizorhabdus wittichii |
1.2.5.2 | Swit_4395 | - |
Rhizorhabdus wittichii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.5.2 | 30 | - |
- |
Rhizorhabdus wittichii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.5.2 | 9.5 | - |
- |
Rhizorhabdus wittichii |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.2.5.2 | 9.5 | - |
half-life below 5 min | Rhizorhabdus wittichii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.2.5.2 | physiological function | overproduction of enzyme Swit_4395 in Sphongomonas wittichii RW1 dramatically increases the tolerance of the bacterium toward butyraldehyde and thus might contribute to the detoxification of toxic aldehydes | Rhizorhabdus wittichii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.5.2 | 43 | - |
Butanal | pH 8, 25°C | Rhizorhabdus wittichii |