EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.132 | chitosan | substrate inhibition with Ki of 01.66 mg/ml | Paenibacillus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 40000 | - |
x * 40000, SDS-PAGE | Paenibacillus sp. |
3.2.1.132 | 41800 | - |
x * 41800, calculated from amino acid sequence | Paenibacillus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.132 | chitosan + H2O | Paenibacillus sp. | - |
? | - |
? | |
3.2.1.132 | chitosan + H2O | Paenibacillus sp. 1794 | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.132 | Paenibacillus sp. | J7G4R4 | - |
- |
3.2.1.132 | Paenibacillus sp. 1794 | J7G4R4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.132 | Q-Sepharose column chromatography and hydroxyapatite column chromatography | Paenibacillus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.132 | carboxymethylcellulose + H2O | 58% activity compared to chitosan | Paenibacillus sp. | ? | - |
? | |
3.2.1.132 | carboxymethylcellulose + H2O | 58% activity compared to chitosan | Paenibacillus sp. 1794 | ? | - |
? | |
3.2.1.132 | chitosan + H2O | - |
Paenibacillus sp. | ? | - |
? | |
3.2.1.132 | chitosan + H2O | - |
Paenibacillus sp. 1794 | ? | - |
? | |
3.2.1.132 | methylcellulose + H2O | 14% activity compared to chitosan | Paenibacillus sp. | ? | - |
? | |
3.2.1.132 | methylcellulose + H2O | 14% activity compared to chitosan | Paenibacillus sp. 1794 | ? | - |
? | |
3.2.1.132 | additional information | does not hydrolyze microcrystalline cellulose, chitin, xylan, laminarin, pustulan, pachyman, mannan, or beta-1,3-1,4-glucan | Paenibacillus sp. | ? | - |
? | |
3.2.1.132 | additional information | does not hydrolyze microcrystalline cellulose, chitin, xylan, laminarin, pustulan, pachyman, mannan, or beta-1,3-1,4-glucan | Paenibacillus sp. 1794 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.132 | ? | x * 40000, SDS-PAGE | Paenibacillus sp. |
3.2.1.132 | ? | x * 41800, calculated from amino acid sequence | Paenibacillus sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.132 | Csn | - |
Paenibacillus sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 70 | 85 | the half-life of the enzyme at 70°C in the presence of chitosan substrate is more than 20 h. Maximal activity is observed at 80°C for 10 min and at 85°C for 5 and 3 min incubation times | Paenibacillus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.132 | 126.5 | - |
chitosan | at pH 4.8 and 37°C | Paenibacillus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 4.7 | 4.8 | - |
Paenibacillus sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 3.8 | 6.5 | the enzyme keeps at least 80% of activity between pH 3.8 and 6.5 | Paenibacillus sp. |