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Literature summary extracted from

  • Sakamoto, T.; Inui, M.; Yasui, K.; Hosokawa, S.; Ihara, H.
    Substrate specificity and gene expression of two Penicillium chrysogenum alpha-L-arabinofuranosidases (AFQ1 and AFS1) belonging to glycoside hydrolase families 51 and 54 (2013), Appl. Microbiol. Biotechnol., 97, 1121-1130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 gene afq1, overexpression of the enzyme containing thioredoxin, a histidine tag and a S-tag, attached to the N-terminus in Escherichia coli strain BL21(DE3) Penicillium chrysogenum
3.2.1.55 gene afs1, overexpression of the enzyme containing thioredoxin, a His-tag and a S-tag, attached to the N-terminus in Escherichia coli strain BL21(DE3) Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 49576
-
 x * 71000, recombinant tagged enzyme, SDS-PAGE, x * 52000 untagged enzyme, SDS-PAGE, x * 49576, enzyme residues 27-506, sequence calculation Penicillium chrysogenum
3.2.1.55 52000
-
 x * 71000, recombinant tagged enzyme, SDS-PAGE, x * 52000 untagged enzyme, SDS-PAGE, x * 49576, enzyme residues 27-506, sequence calculation Penicillium chrysogenum
3.2.1.55 67496
-
 x * 85000, recombinant tagged enzyme, SDS-PAGE, x * 79000, untagged enzyme, SDS-PAGE, x * 67496, enzyme residues 18-635, sequence calculation Penicillium chrysogenum
3.2.1.55 71000
-
 x * 71000, recombinant tagged enzyme, SDS-PAGE, x * 52000 untagged enzyme, SDS-PAGE, x * 49576, enzyme residues 27-506, sequence calculation Penicillium chrysogenum
3.2.1.55 79000
-
 x * 85000, recombinant tagged enzyme, SDS-PAGE, x * 79000, untagged enzyme, SDS-PAGE, x * 67496, enzyme residues 18-635, sequence calculation Penicillium chrysogenum
3.2.1.55 85000
-
 x * 85000, recombinant tagged enzyme, SDS-PAGE, x * 79000, untagged enzyme, SDS-PAGE, x * 67496, enzyme residues 18-635, sequence calculation Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Penicillium chrysogenum B5MGR0 constitutive gene afq1
-
3.2.1.55 Penicillium chrysogenum B5MGR1 inducible gene afs1
-
3.2.1.55 Penicillium chrysogenum 31B B5MGR0 constitutive gene afq1
-
3.2.1.55 Penicillium chrysogenum 31B B5MGR1 inducible gene afs1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.55 glycoprotein 7 asparagine-linked oligosaccharides for enzyme AFQ1 Penicillium chrysogenum
3.2.1.55 glycoprotein one asparagine-linked oligosaccharide for enzyme AFS1 Penicillium chrysogenum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Penicillium chrysogenum 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Penicillium chrysogenum 31B 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 debranched arabinan + H2O
-
 Penicillium chrysogenum ?
-
 ?
3.2.1.55 debranched arabinan + H2O
-
 Penicillium chrysogenum 31B ?
-
 ?
3.2.1.55 additional information substrate specificity, overview. The recombinant enzyme degrades arabinofuranosyl side chains that are both singly and doubly linked to the backbones of arabinoxylan and L-arabinan, NMR spectroscopic analysis Penicillium chrysogenum ?
-
 ?
3.2.1.55 additional information substrate specificity, overview. The recombinant enzyme preferentially releases arabinose linked to C-3 of single-substituted xylose or arabinose residues in the two polysaccharides, NMR spectroscopic analysis Penicillium chrysogenum ?
-
 ?
3.2.1.55 additional information substrate specificity, overview. The recombinant enzyme preferentially releases arabinose linked to C-3 of single-substituted xylose or arabinose residues in the two polysaccharides, NMR spectroscopic analysis Penicillium chrysogenum 31B ?
-
 ?
3.2.1.55 additional information substrate specificity, overview. The recombinant enzyme degrades arabinofuranosyl side chains that are both singly and doubly linked to the backbones of arabinoxylan and L-arabinan, NMR spectroscopic analysis Penicillium chrysogenum 31B ?
-
 ?
3.2.1.55 sugar beet L-arabinan + H2O
-
 Penicillium chrysogenum ?
-
 ?
3.2.1.55 sugar beet L-arabinan + H2O
-
 Penicillium chrysogenum 31B ?
-
 ?
3.2.1.55 wheat arabinoxylan + H2O
-
 Penicillium chrysogenum ?
-
 ?
3.2.1.55 wheat arabinoxylan + H2O
-
 Penicillium chrysogenum 31B ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 ? x * 71000, recombinant tagged enzyme, SDS-PAGE, x * 52000 untagged enzyme, SDS-PAGE, x * 49576, enzyme residues 27-506, sequence calculation Penicillium chrysogenum
3.2.1.55 ? x * 85000, recombinant tagged enzyme, SDS-PAGE, x * 79000, untagged enzyme, SDS-PAGE, x * 67496, enzyme residues 18-635, sequence calculation Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 AFQ1
-
 Penicillium chrysogenum
3.2.1.55 AFS1
-
 Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 50
-
-
 Penicillium chrysogenum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 50
-
 purified recombinant enzyme, pH 5.0, stable up to Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5
-
 assay at Penicillium chrysogenum

Expression

EC Number Organism Comment Expression
3.2.1.55 Penicillium chrysogenum gene afq1 is constitutive additional information
3.2.1.55 Penicillium chrysogenum gene afs1 is strongly induced by arabinose, arabinitol, arabinan, and arabinoxylan, and by galactan to a lesser extent up

General Information

EC Number General Information Comment Organism
3.2.1.55 evolution the enzyme belongs to the family 51 glycoside hydrolase, GH51 Penicillium chrysogenum
3.2.1.55 evolution the enzyme belongs to the family 54 glycoside hydrolase, GH54 Penicillium chrysogenum