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Literature summary extracted from

  • Hong, P.Y.; Iakiviak, M.; Dodd, D.; Zhang, M.; Mackie, R.I.; Cann, I.
    Two new xylanases with different substrate specificities from the human gut bacterium Bacteroides intestinalis DSM 17393 (2014), Appl. Environ. Microbiol., 80, 2084-2093.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.156 gene rex8A, the gene is located downstream of a GH3 gene (xyl3A), DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL-21 Codon-Plus(DE3) RIL Bacteroides intestinalis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.156 D148A site-directed mutagenesis, inactive mutant Bacteroides intestinalis
3.2.1.156 D286A site-directed mutagenesis, inactive mutant Bacteroides intestinalis
3.2.1.156 E90A site-directed mutagenesis, inactive mutant Bacteroides intestinalis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.156 Bacteroides intestinalis
-
 gene rex8A
-
3.2.1.156 Bacteroides intestinalis DSM 17393
-
 gene rex8A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.156 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL-21 Codon-Plus(DE3) RIL by metal affinity chromatography Bacteroides intestinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.156 additional information the enzyme hydrolyzes xylotriose through xylohexaose to a mixture of xylose and xylobiose, identification by thin layer chromatography Bacteroides intestinalis ?
-
 ?
3.2.1.156 additional information the enzyme hydrolyzes xylotriose through xylohexaose to a mixture of xylose and xylobiose, identification by thin layer chromatography Bacteroides intestinalis DSM 17393 ?
-
 ?
3.2.1.156 xylohexaose + H2O
-
 Bacteroides intestinalis xylose + xylobiose
-
 ?
3.2.1.156 xylohexaose + H2O
-
 Bacteroides intestinalis DSM 17393 xylose + xylobiose
-
 ?
3.2.1.156 xylopentaose + H2O
-
 Bacteroides intestinalis xylose + xylobiose
-
 ?
3.2.1.156 xylopentaose + H2O
-
 Bacteroides intestinalis DSM 17393 xylose + xylobiose
-
 ?
3.2.1.156 xylotetraose + H2O
-
 Bacteroides intestinalis xylose + xylobiose
-
 ?
3.2.1.156 xylotetraose + H2O
-
 Bacteroides intestinalis DSM 17393 xylose + xylobiose
-
 ?
3.2.1.156 xylotriose + H2O
-
 Bacteroides intestinalis xylose + xylobiose
-
 ?
3.2.1.156 xylotriose + H2O
-
 Bacteroides intestinalis DSM 17393 xylose + xylobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.156 More secondary and quarternary structure analysis, gel filtration and circular dichroism spectroscopy, overview Bacteroides intestinalis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.156 GH8 xylanase
-
 Bacteroides intestinalis
3.2.1.156 reducing-end xylose-releasing exo-oligoxylanase
-
 Bacteroides intestinalis
3.2.1.156 Rex8A
-
 Bacteroides intestinalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.156 37
-
 assay at Bacteroides intestinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.156 6
-
 assay at Bacteroides intestinalis

General Information

EC Number General Information Comment Organism
3.2.1.156 additional information three conserved catalytic residues are Glu90, Asp148, and Asp286 Bacteroides intestinalis