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Literature summary extracted from

  • Hess, V.; Gonzalez, J.M.; Parthasarathy, A.; Buckel, W.; Müller, V.
    Caffeate respiration in the acetogenic bacterium Acetobacterium woodii: a coenzyme A loop saves energy for caffeate activation (2013), Appl. Environ. Microbiol., 79, 1942-1947.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.23 overproduction in Escherichia coli Acetobacterium woodii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.3.23 0.008
-
 3,4-dihydroxyphenylpropanoyl-CoA pH 7.5, 40°C Acetobacterium woodii
2.8.3.23 0.075
-
 (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate pH 7.5, 40°C Acetobacterium woodii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.23 60000
-
 2 * 60000, SDS-PAGE Acetobacterium woodii
2.8.3.23 130000
-
 gel filtration Acetobacterium woodii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.23 3,4-dihydroxyphenylpropanoyl-CoA + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate Acetobacterium woodii the enzyme catalyzes an energy-saving CoA loop for caffeate activation. 3-(3,4-dihydroxyphenyl)acrylate i.e. caffeate 3,4-dihydroxyphenylpropanoate + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.23 Acetobacterium woodii F1CYZ5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.23
-
 Acetobacterium woodii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.3.23 3-(3,4-dihydroxyphenyl)propanoyl-CoA + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate = 3-(3,4-dihydroxyphenyl)propanoate + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA the enzyme uses a ping-pong mechanism for CoA transfer, it has two catalytic domains on one polypeptide chain. (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate = caffeate. 3,4-dihydroxyphenylpropanoate = hydrocaffeate Acetobacterium woodii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.23 3,4-dihydroxyphenylpropanoyl-CoA + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate the enzyme catalyzes an energy-saving CoA loop for caffeate activation. 3-(3,4-dihydroxyphenyl)acrylate i.e. caffeate Acetobacterium woodii 3,4-dihydroxyphenylpropanoate + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA
-
 ?
2.8.3.23 3,4-dihydroxyphenylpropanoyl-CoA + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate 84% compared to the activity with 4-coumarate. (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate i.e. caffeate. 3,4-dihydroxyphenylpropanoate i.e. hydrocaffeate Acetobacterium woodii 3,4-dihydroxyphenylpropanoate + (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA
-
 ?
2.8.3.23 3,4-dihydroxyphenylpropanoyl-CoA + 4-coumarate 3,4-dihydroxyphenylpropanoyl-CoA i.e. hydrocaffeoyl-CoA Acetobacterium woodii 3,4-dihydroxyphenylpropanoate + 4-coumaroyl-CoA
-
 ?
2.8.3.23 3,4-dihydroxyphenylpropanoyl-CoA + ferulate 86% compared to the activity with 4-coumarate Acetobacterium woodii 3,4-dihydroxyphenylpropanoate + feruloyl-CoA
-
 ?
2.8.3.23 additional information no activity with cinnamate, sinapate, or 4-hydroxybenzoate as a CoA acceptor. Neither acetyl-CoA nor butanoyl-CoA serve as the CoA donor for the enzyme Acetobacterium woodii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.23 homodimer 2 * 60000, SDS-PAGE Acetobacterium woodii

Synonyms

EC Number Synonyms Comment Organism
2.8.3.23 CarA
-
 Acetobacterium woodii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.23 40
-
 assay at Acetobacterium woodii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.3.23 0.008
-
 3,4-dihydroxyphenylpropanoyl-CoA pH 7.5, 40°C Acetobacterium woodii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.3.23 7.5
-
 assay at Acetobacterium woodii