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Literature summary extracted from

  • Ichinose, H.; Araki, Y.; Michikawa, M.; Harazono, K.; Yaoi, K.; Karita, S.; Kaneko, S.
    Characterization of an endo-processive-type xyloglucanase having a beta-1,4-glucan-binding module and an endo-type xyloglucanase from Streptomyces avermitilis (2012), Appl. Environ. Microbiol., 78, 7939-7945.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.151 gene sav_1856, DNA and amino acid sequence determination and analysis Streptomyces avermitilis
3.2.1.151 gene sav_2574, DNA and amino acid sequence determination and analysis Streptomyces avermitilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.151 extracellular
-
Streptomyces avermitilis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.151 78000
-
x * 78000, recombinant enzyme, SDS.PAGE Streptomyces avermitilis
3.2.1.151 92000
-
x * 92000, recombinant enzyme, SDS-PAGE Streptomyces avermitilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.151 Streptomyces avermitilis Q82K30 gene sav_2574
-
3.2.1.151 Streptomyces avermitilis Q82M04 gene sav_1856
-
3.2.1.151 Streptomyces avermitilis DSM 46492 Q82K30 gene sav_2574
-
3.2.1.151 Streptomyces avermitilis NBRC 14893 Q82M04 gene sav_1856
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.151 additional information enzyme SaGH74A is endo-processive, hydrolysis pattern and mode of action, overview. The C-terminus of enzyme SaGH74A, which is annotated as a carbohydrate-binding module family 2 (CBM2), binds to beta-1,4-linked glucan-containing soluble polysaccharides such as hydroxyethyl cellulose, barley glucan, and xyloglucan, affinities, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan) Streptomyces avermitilis ?
-
?
3.2.1.151 additional information enzyme SaGH74B is a typical endo-enzyme, hydrolysis pattern and mode of action, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan) Streptomyces avermitilis ?
-
?
3.2.1.151 additional information enzyme SaGH74A is endo-processive, hydrolysis pattern and mode of action, overview. The C-terminus of enzyme SaGH74A, which is annotated as a carbohydrate-binding module family 2 (CBM2), binds to beta-1,4-linked glucan-containing soluble polysaccharides such as hydroxyethyl cellulose, barley glucan, and xyloglucan, affinities, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan) Streptomyces avermitilis NBRC 14893 ?
-
?
3.2.1.151 additional information enzyme SaGH74B is a typical endo-enzyme, hydrolysis pattern and mode of action, overview. No activity for Avicel (crystalline cellulose), Celish (microfibers), carboxymethyl cellulose (beta-1,4-glucan), barley beta-glycan (beta-1,3-beta-1,4-glucan), and lichenan (beta-1,3-beta-1,4-glucan) Streptomyces avermitilis DSM 46492 ?
-
?
3.2.1.151 tamarind xyloglucan + H2O
-
Streptomyces avermitilis ?
-
?
3.2.1.151 tamarind xyloglucan + H2O
-
Streptomyces avermitilis NBRC 14893 ?
-
?
3.2.1.151 tamarind xyloglucan + H2O
-
Streptomyces avermitilis DSM 46492 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.151 ? x * 78000, recombinant enzyme, SDS.PAGE Streptomyces avermitilis
3.2.1.151 ? x * 92000, recombinant enzyme, SDS-PAGE Streptomyces avermitilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.151 SaGH74A
-
Streptomyces avermitilis
3.2.1.151 SaGH74B
-
Streptomyces avermitilis
3.2.1.151 xyloglucanase
-
Streptomyces avermitilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.151 55
-
recombinant enzyme Streptomyces avermitilis
3.2.1.151 60
-
recombinant enzyme Streptomyces avermitilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.151 30 60 activity range, rapid loss of activity above 60°C, profile overview Streptomyces avermitilis
3.2.1.151 30 75 activity range, profile overview Streptomyces avermitilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.151 10 50 purified recombinant enzyme, stable at, rapid loss of activity above 50°C, profile overview Streptomyces avermitilis
3.2.1.151 30
-
purified recombinant enzyme, stable up to Streptomyces avermitilis
3.2.1.151 40
-
purified recombinant enzyme, inactivation Streptomyces avermitilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 5.5
-
recombinant enzyme Streptomyces avermitilis
3.2.1.151 6
-
recombinant enzyme Streptomyces avermitilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.151 3.5 9.5 activity range, profile overview Streptomyces avermitilis
3.2.1.151 4 7 activity range, profile overview Streptomyces avermitilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.151 4.5 9 purified recombinant enzyme, stable at Streptomyces avermitilis
3.2.1.151 6 6.5 purified recombinant enzyme, stable at Streptomyces avermitilis

General Information

EC Number General Information Comment Organism
3.2.1.151 evolution the enzyme belongs to the glycoside hydrolase family 74, GH74 Streptomyces avermitilis