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Literature summary extracted from

  • Nakajima, A.; Aono, T.; Tsukada, S.; Siarot, L.; Ogawa, T.; Oyaizu, H.
    Lon protease of Azorhizobium caulinodans ORS571 is required for suppression of reb gene expression (2012), Appl. Environ. Microbiol., 78, 6251-6261.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.53 recombinant expression of N-terminally His6-tagged wild-type and mutant S684A Lon in Escherichia coli strain BLR-DE3 Azorhizobium caulinodans

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.53 additional information knockout mutant generation by lon gene deletion, generation of a lon/reb double knockout mutant Azorhizobium caulinodans
3.4.21.53 S684A site-directed mutagenesis, catalytic site mutant Azorhizobium caulinodans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.53 Mg2+ required Azorhizobium caulinodans

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.53 Azorhizobium caulinodans A8HYF7 gene lon
-
3.4.21.53 Azorhizobium caulinodans ORS 571 A8HYF7 gene lon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.53 recombinant N-terminally His6-tagged wild-type and mutant S684A Lon from Escherichia coli strain BLR-DE3 by nickel affinity chromatography Azorhizobium caulinodans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.53 additional information two kinds of host cells are observed in the stem nodules of host legume Sesbania rostrata Azorhizobium caulinodans
-

Synonyms

EC Number Synonyms Comment Organism
3.4.21.53 lon protease
-
 Azorhizobium caulinodans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.53 37
-
 assay at Azorhizobium caulinodans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.53 7.6
-
 assay at Azorhizobium caulinodans

General Information

EC Number General Information Comment Organism
3.4.21.53 malfunction the stem nodules in the host legume Sesbania rostrata formed by the lon mutant show little or no nitrogen fixation activity. The reb genes are highly expressed in the lon mutant, high expression of reb genes in part causes aberrance in the Azorhizobium caulinodans-Sesbania rostrata symbiosis Azorhizobium caulinodans
3.4.21.53 physiological function Lon protease is required to suppress the expression of the reb genes. Lon protease is also involved in the regulation of exopolysaccharide production and autoagglutination of bacterial cells Azorhizobium caulinodans