Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chi, W.J.; Park, J.S.; Kang, D.K.; Hong, S.K.
    Production and characterization of a novel thermostable extracellular agarase from Pseudoalteromonas hodoensis newly isolated from the West Sea of South Korea (2014), Appl. Biochem. Biotechnol., 173, 1703-1716.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.B1 extracellular
-
 Pseudoalteromonas hodoensis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B1 35000
-
 x * 35000, SDS-PAGE Pseudoalteromonas hodoensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.B1 agarose + H2O Pseudoalteromonas hodoensis
-
 neoagarotetraose + neoagarohexaose + neoagarooctaose neoagarotetraose and neoagarohexaose are main products, while neoagarooctaose is a minor product ?
3.2.1.B1 agarose + H2O Pseudoalteromonas hodoensis H7T
-
 neoagarotetraose + neoagarohexaose + neoagarooctaose neoagarotetraose and neoagarohexaose are main products, while neoagarooctaose is a minor product ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B1 Pseudoalteromonas hodoensis
-
 DSM25967T
-
3.2.1.B1 Pseudoalteromonas hodoensis H7T
-
 DSM25967T
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B1 ammonium sulfate precipitation, Resource Q column chromatography, and Superdex 200 gel filtration Pseudoalteromonas hodoensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B1 4-nitrophenyl alpha-D-galactopyranoside + H2O negligible activity Pseudoalteromonas hodoensis 4-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.B1 4-nitrophenyl alpha-D-galactopyranoside + H2O negligible activity Pseudoalteromonas hodoensis H7T 4-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.B1 4-nitrophenyl beta-D-galactopyranoside + H2O strong hydrolytic activity Pseudoalteromonas hodoensis 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B1 4-nitrophenyl beta-D-galactopyranoside + H2O strong hydrolytic activity Pseudoalteromonas hodoensis H7T 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B1 agarose + H2O
-
 Pseudoalteromonas hodoensis neoagarotetraose + neoagarohexaose + neoagarooctaose neoagarotetraose and neoagarohexaose are main products, while neoagarooctaose is a minor product ?
3.2.1.B1 agarose + H2O
-
 Pseudoalteromonas hodoensis H7T neoagarotetraose + neoagarohexaose + neoagarooctaose neoagarotetraose and neoagarohexaose are main products, while neoagarooctaose is a minor product ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B1 ? x * 35000, SDS-PAGE Pseudoalteromonas hodoensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B1 AgaA7
-
 Pseudoalteromonas hodoensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B1 45
-
-
 Pseudoalteromonas hodoensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B1 40 60 approximately 90 and 60% of the maximum activity is detected at 50 and 40 °C, respectively, but drastic reduction of activity (50%) is observed at 60°C Pseudoalteromonas hodoensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B1 7
-
 at 45°C Pseudoalteromonas hodoensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B1 8 10 above 90% activity is obtained at pH 8.0 and 9.0. Less than 60% of the maximum activity is detected at pH 10.0 Pseudoalteromonas hodoensis