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Literature summary extracted from

  • Chiyanzu, I.; Brienzo, M.; Garcia-Aparicio, M.P.; Goergens, J.F.
    Application of endo-beta-1,4,D-mannanase and cellulase for the release of mannooligosaccharides from steam-pretreated spent coffee ground (2014), Appl. Biochem. Biotechnol., 172, 3538-3557.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 gene manA, recombinant expression in and secretion from Yarrowia lipolytica Aspergillus aculeatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.78 additional information Aspergillus aculeatus the enzyme is involved in release of mannooligosaccharides from spent coffee ground cleaving the backbone at random locations in galactomannan, glucomannan, galactoglucomannan and mannan ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Aspergillus aculeatus
-
 gene manA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 recombinant extracellular enzyme from Yarrowia lipolytica culture supernatant by ultrafiltration Aspergillus aculeatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 additional information
-
 recombinant enzyme in Yarrowia lipolytica is produced with an activity of 183.5 U/ml and 0.23 mg protein/ml Aspergillus aculeatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 locust bean gum + H2O
-
 Aspergillus aculeatus ?
-
 ?
3.2.1.78 additional information the enzyme is involved in release of mannooligosaccharides from spent coffee ground cleaving the backbone at random locations in galactomannan, glucomannan, galactoglucomannan and mannan Aspergillus aculeatus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 endo-beta-1,4,D-mannanase
-
 Aspergillus aculeatus
3.2.1.78 ManA
-
 Aspergillus aculeatus
3.2.1.78 ManA/HmA
-
 Aspergillus aculeatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 80
-
 recombinant enzyme Aspergillus aculeatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.78 30 90 activity range, profile overview Aspergillus aculeatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 additional information
-
 glycerol at 30-40%, potassium sorbate/sodium benzoate at 0.2%, and sorbitol at 30-35% enhances enzyme thermostability and activity, overview Aspergillus aculeatus
3.2.1.78 60
-
 a combination of mannanase with 0.2% sodium benzoate and 30% or 35% sorbitol presents the highest relative activity of about 150% when incubated at 60°C for 4 h, the control retains 63% of the initial activity Aspergillus aculeatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 4.8
-
 assay at Aspergillus aculeatus

Expression

EC Number Organism Comment Expression
3.2.1.78 Aspergillus aculeatus recombinant enzyme production is improved by 150% via supplementation with 0.2% sodium benzoate and 35% sorbitol as a preservative and stabiliser, respectively up