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Literature summary extracted from

  • Juarez-Montiel, M.; Ibarra, J.A.; Chavez-Camarillo, G.; Hernandez-Rodriguez, C.; Villa-Tanaca, L.
    Molecular cloning and heterologous expression in Pichia pastoris of X-prolyl-dipeptidyl aminopeptidase from basidiomycete Ustilago maydis (2014), Appl. Biochem. Biotechnol., 172, 2530-2539.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.9 expressed in Pichia pastoris Ustilago maydis
3.4.14.5 expressed in Pichia pastoris Ustilago maydis
3.4.14.11 expression in Pichia pastoris Ustilago maydis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.9 pefabloc
-
 Ustilago maydis
3.4.11.9 phenylmethylsulfonyl fluoride
-
 Ustilago maydis
3.4.14.5 pefabloc
-
 Ustilago maydis
3.4.14.5 phenylmethylsulfonyl fluoride
-
 Ustilago maydis
3.4.14.11 pefabloc 1 mM, 35% residual activity Ustilago maydis
3.4.14.11 PMSF 1 mM, 8.4% residual activity Ustilago maydis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.9 125000
-
 SDS-PAGE Ustilago maydis
3.4.14.5 125000
-
 SDS-PAGE Ustilago maydis
3.4.14.11 125000
-
 x * 125000, SDS-PAGE of recombinant protein Ustilago maydis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.9 Ustilago maydis
-
-
-
3.4.14.5 Ustilago maydis
-
-
-
3.4.14.11 Ustilago maydis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.9 Ni-NTA affinity chromatography Ustilago maydis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.9 Ala-Pro-4-nitroanilide + H2O
-
 Ustilago maydis Ala + Pro-4-nitroanilide
-
 ?
3.4.14.5 L-Pro-4-nitroanilide + H2O
-
 Ustilago maydis L-Pro + 4-nitroaniline
-
 ?
3.4.14.11 Gly-L-Pro 4-nitroanilide + H2O 16% of the activity with L-Ala-L-Pro 4-nitroanilide Ustilago maydis Gly-L-Pro + 4-nitroaniline
-
 ?
3.4.14.11 L-Ala-L-Pro 4-nitroanilide + H2O
-
 Ustilago maydis L-Ala-L-Pro + 4-nitroaniline
-
 ?
3.4.14.11 L-Arg-L-Pro 4-nitroanilide + H2O 13.6% of the activity with L-Ala-L-Pro 4-nitroanilide Ustilago maydis L-Arg-L-Pro + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.11 ? x * 125000, SDS-PAGE of recombinant protein Ustilago maydis

Synonyms

EC Number Synonyms Comment Organism
3.4.11.9 dapUm
-
 Ustilago maydis
3.4.11.9 X-prolyl-dipeptidyl aminopeptidase
-
 Ustilago maydis
3.4.14.5 dapUm
-
 Ustilago maydis
3.4.14.5 X-prolyl-dipeptidyl aminopeptidase
-
 Ustilago maydis
3.4.14.11 DAP
-
 Ustilago maydis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.9 40
-
 recombinant protein Ustilago maydis
3.4.14.5 40
-
-
 Ustilago maydis
3.4.14.11 35
-
-
 Ustilago maydis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.9 5 40 stable for 60 min Ustilago maydis
3.4.14.5 5 40 stable for 60 min Ustilago maydis
3.4.14.11 5 40 stable for 60 min Ustilago maydis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.9 6.9
-
 native protein Ustilago maydis
3.4.11.9 7
-
 recombinant protein Ustilago maydis
3.4.14.5 6
-
 recombinant protein Ustilago maydis
3.4.14.5 6.9
-
 native protein Ustilago maydis
3.4.14.11 7
-
-
 Ustilago maydis