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Literature summary extracted from

  • Tomita, M.; Kikuchi, A.; Kobayashi, M.; Yamaguchi, M.; Ifuku, S.; Yamashoji, S.; Ando, A.; Saito, A.
    Characterization of antifungal activity of the GH-46 subclass III chitosanase from Bacillus circulans MH-K1 (2013), Antonie van Leeuwenhoek, 104, 737-748.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 expressed in Escherichia coli BL21(DE3) cells Niallia circulans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.132 E37Q the mutation abolishes the antifungal activity of chitosanase Niallia circulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Niallia circulans
-
 ?
-
 ?
3.2.1.132 chitosan + H2O Niallia circulans MH-K1
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Niallia circulans
-
-
-
3.2.1.132 Niallia circulans MH-K1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 CM-Toyopearl 650 M column chromatography and Resource S column chromatography Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Niallia circulans MH-K1 ?
-
 ?

General Information

EC Number General Information Comment Organism
3.2.1.132 physiological function citosanase inhibits the growth of zygomycetes fungi Rhizopus and Mucor probably via its fungistatic effect Niallia circulans