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Literature summary extracted from

  • Stressler, T.; Eisele, T.; Schlayer, M.; Fischer, L.
    Production, active staining and gas chromatography assay analysis of recombinant aminopeptidase P from Lactococcus lactis ssp. lactis DSM 20481 (2012), AMB Express, 2, 39.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.9 expressed in Escherichia coli as a His-tagged fusion protein Lactococcus lactis subsp. lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.9 1,10-phenanthroline
-
 Lactococcus lactis subsp. lactis
3.4.11.9 EDTA
-
 Lactococcus lactis subsp. lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.9 2.5
-
 L-Ile-L-Pro-L-Pro pH 7, temperature not specified in the publication Lactococcus lactis subsp. lactis
3.4.11.9 4.7
-
 L-Leu-L-Pro-L-Pro pH 7, temperature not specified in the publication Lactococcus lactis subsp. lactis
3.4.11.9 13.6
-
 L-Val-L-Pro-L-Pro pH 7, temperature not specified in the publication Lactococcus lactis subsp. lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.9 Co2+ 10 mM, moderate inhibitory effect Lactococcus lactis subsp. lactis
3.4.11.9 Cu2+ 0.1 mM, moderate inhibitory effect Lactococcus lactis subsp. lactis
3.4.11.9 Cu2+ 10 mM, strong inhibitory effect Lactococcus lactis subsp. lactis
3.4.11.9 Mn2+ 8fold increase Lactococcus lactis subsp. lactis
3.4.11.9 Sn2+ 10 mM, strong inhibitory effect Lactococcus lactis subsp. lactis
3.4.11.9 Zn2+ 0.01 mM, moderate inhibitory effect Lactococcus lactis subsp. lactis
3.4.11.9 Zn2+ 10 mM, strong inhibitory effect Lactococcus lactis subsp. lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.9 40000
-
 native PAGE, SDS-PAGE Lactococcus lactis subsp. lactis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.9 Lactococcus lactis subsp. lactis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.9 using His-tag affinity chromatography and desalting Lactococcus lactis subsp. lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.9 L-Ile-L-Pro-L-Pro + H2O
-
 Lactococcus lactis subsp. lactis L-Ile + L-Pro-L-Pro
-
 ?
3.4.11.9 L-Leu-L-Pro-L-Pro + H2O
-
 Lactococcus lactis subsp. lactis L-Leu + L-Pro-L-Pro
-
 ?
3.4.11.9 L-Val-L-Pro-L-Pro + H2O
-
 Lactococcus lactis subsp. lactis L-Val + L-Pro-L-Pro
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.9 aminopeptidase P
-
 Lactococcus lactis subsp. lactis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.9 50 60
-
 Lactococcus lactis subsp. lactis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.9
-
 37 reasonable stability at 0°C, 20°C and 37°C over 15 days Lactococcus lactis subsp. lactis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.9 7 7.5
-
 Lactococcus lactis subsp. lactis