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Literature summary extracted from

  • He, M.; Zheng, Y.; Huang, C.H.; Qian, G.; Xiao, X.; Ko, T.P.; Shao, W.; Guo, R.T.
    Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) from Thermotoga maritima (2014), Acta Crystallogr. Sect. F, 70, 1563-1565.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.2.1 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.13.2.1 sitting drop vapor diffusion method, using 0.3 M calcium acetate hydrate, 0.1 M sodium cacodylate trihydrate pH 6.5, and 8% (w/v) polyethylene glycol 8000 Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.2.1 S-adenosyl-L-homocysteine + H2O Thermotoga maritima
-
 L-homocysteine + adenosine
-
 r
3.13.2.1 S-adenosyl-L-homocysteine + H2O Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
 L-homocysteine + adenosine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.13.2.1 Thermotoga maritima O51933
-
-
3.13.2.1 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 O51933
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.2.1 nickel-affinity column chromatography Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.2.1 S-adenosyl-L-homocysteine + H2O
-
 Thermotoga maritima L-homocysteine + adenosine
-
 r
3.13.2.1 S-adenosyl-L-homocysteine + H2O
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 L-homocysteine + adenosine
-
 r

Subunits

EC Number Subunits Comment Organism
3.13.2.1 homotetramer x-ray crystallography Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.13.2.1 S-adenosyl-L-homocysteine hydrolase
-
 Thermotoga maritima
3.13.2.1 SAHH
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.13.2.1 75
-
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.13.2.1 75
-
 the enzyme exhibits extreme thermostability and retains 95% enzyme activity after incubation at 75°C for 2 h Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.13.2.1 6.5
-
-
 Thermotoga maritima