Literature summary extracted from
Moeller, M.S.; Abou Hachem, M.; Svensson, B.; Henriksen, A.
Structure of the starch-debranching enzyme barley limit dextrinase reveals homology of the N-terminal domain to CBM21 (2012), Acta Crystallogr. Sect. F, 68, 1008-1012.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.142 |
recombinant epression in Pichia pastoris |
Hordeum vulgare |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.2.1.142 |
purified free enzyme with a glycerol molecule in the active site or purified enzyme in complex with competitive inhibitors alpha- and beta-cyclodextrin, hanging drop vapour diffusion method, streak-seeding, mixing of 10 mg/mlprotein in 50 mM MES buffer pH 6.6, 250 mM NaCl, 0.5 mM CaCl2, 0.67mM maltotriose, with a reservoir solution consisting of 30% w/v PEG 3350, 5% glycerol, 0.3 M NaI, cysteine is added to the crystallization drops to a final concentration of 5-7 mM, one week, 20°C, X-ray diffraction structure determination and analysis at 1.9-2.5 A resolution, molecular replacement and modelling |
Hordeum vulgare |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.2.1.142 |
alpha-cyclodextrin |
competitive inhibitor |
Hordeum vulgare |
|
3.2.1.142 |
beta-cyclodextrin |
competitive inhibitor |
Hordeum vulgare |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.2.1.142 |
limit dextrin + H2O |
Hordeum vulgare |
the enzyme hydrolyses alpha-1,6-glucosidic linkages in limit dextrins derived from amylopectin |
? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.142 |
Hordeum vulgare |
O48541 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.142 |
limit dextrin + H2O |
the enzyme hydrolyses alpha-1,6-glucosidic linkages in limit dextrins derived from amylopectin |
Hordeum vulgare |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.142 |
starch-debranching enzyme |
- |
Hordeum vulgare |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.2.1.142 |
evolution |
the debranching enzyme belongs to the glycoside hydrolase family 13 subfamily 13, GH13_13 |
Hordeum vulgare |