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Literature summary extracted from

  • Goncalves, A.M.; Silva, C.S.; Madeira, T.I.; Coelho, R.; de Sanctis, D.; San Romao, M.V.; Bento, I.
    Endo-beta-D-1,4-mannanase from Chrysonilia sitophila displays a novel loop arrangement for substrate selectivity (2012), Acta Crystallogr. Sect. D, 68, 1468-1478.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 gene man5, DNA and amino acid sequence determination and analysis Neurospora sitophila

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.78 hanging drop vapour diffusion method, mixing of 6.5 mg/ml protein in 20 mM Tris-HCl, pH 7.6, with reservoir solution containing 0.1 M magnesium chloride, 0.1 M sodium acetate pH 4.5, 23% w/v PEG 3350, 20°C, method optimization, X-ray diffraction structure determination and analysis at 1.40 A resolution, molecular replacement Neurospora sitophila

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.78 additional information mutagenesis of Trp271 at the +1 subsite plays a crucial role in the enhanced transglycosylation activity Neurospora sitophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.78 additional information Neurospora sitophila the native wild-type enzyme shows bifunctional activity as both an endo-glucanase and an endo-mannanase, substrate specificity, overview ?
-
 ?
3.2.1.78 additional information Neurospora sitophila DSM 16514 the native wild-type enzyme shows bifunctional activity as both an endo-glucanase and an endo-mannanase, substrate specificity, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Neurospora sitophila I4IY26 i.e. Neurospora sitophila, gene man5
-
3.2.1.78 Neurospora sitophila DSM 16514 I4IY26 i.e. Neurospora sitophila, gene man5
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 glycoprotein the enzyme shows two different N-glycosylation sites. The first site Asn63 contains two linked N-acetylglucosamine residues. This glycosylation may have a structural role in stabilizing structural elements, as there are hydrogen bonds between the glucosamine residues and Ile67 and Leu71, which both belong to a very long loop (residues 64-84), and Val136 located on helix 4. The other site Asn319 is not fully occupied, suggesting disorder or perhaps different populations of glycosylation Neurospora sitophila

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 native enzyme by ultrafiltration and anion exchange chromatography Neurospora sitophila

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.78 additional information solid state fermentation on medium containing microcrystalline cellulose Neurospora sitophila
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Neurospora sitophila 4-nitrophenol + beta-D-mannopyranose
-
 ?
3.2.1.78 4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Neurospora sitophila DSM 16514 4-nitrophenol + beta-D-mannopyranose
-
 ?
3.2.1.78 azo-carob galactomannan + H2O
-
 Neurospora sitophila ?
-
 ?
3.2.1.78 azo-carob galactomannan + H2O
-
 Neurospora sitophila DSM 16514 ?
-
 ?
3.2.1.78 additional information the native wild-type enzyme shows bifunctional activity as both an endo-glucanase and an endo-mannanase, substrate specificity, overview Neurospora sitophila ?
-
 ?
3.2.1.78 additional information the enzyme also show transglycosylation activity Neurospora sitophila ?
-
 ?
3.2.1.78 additional information the native wild-type enzyme shows bifunctional activity as both an endo-glucanase and an endo-mannanase, substrate specificity, overview Neurospora sitophila DSM 16514 ?
-
 ?
3.2.1.78 additional information the enzyme also show transglycosylation activity Neurospora sitophila DSM 16514 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 More the enzyme adopts the (beta/alpha)8-barrel fold Neurospora sitophila

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 CsMan5
-
 Neurospora sitophila
3.2.1.78 endo-beta-D-1,4-mannanase
-
 Neurospora sitophila

General Information

EC Number General Information Comment Organism
3.2.1.78 evolution the enzyme belongs to the glycosyl hydrolase family 5, GH5, structure comparisons, the overall fold of the enzyme is strongly conserved, overview. The enzyme displays the typical (beta/alpha)8-barrel fold and a unique structural arrangement of three surface loops that stretch over the active centre, promoting an altered topography of the binding cleft Neurospora sitophila
3.2.1.78 additional information tertiary structure, active site and substrate binding site structures analysis, detailed overview. Two tryptophan residues that provide the hydrophobic stacking of the +1 subsite Trp125 and Trp271,is 9.5 A, making bulkier branched substrates difficult to accommodate Neurospora sitophila