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Literature summary extracted from

  • Liu, S.; Zhang, C.; Li, N.; Niu, B.; Liu, M.; Liu, X.; Wie, T.; Zhu, D.; Huang, Y.; Xu, S.; Gu, L.
    Structural insight into the ISC domain of VibB from Vibrio cholerae at atomic resolution: a snapshot just before the enzymatic reaction (2012), Acta Crystallogr. Sect. D, 68, 1329-1338.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.3.2.1 expressed in Escherichia coli BL21(DE3) cells Vibrio cholerae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.3.2.1 sitting drop vapor diffusion method, using 50 mM CaCl2, 0.1 M bis-Tris pH 6.5, 28% (w/v) polyethylene glycol monomethyl ether 550, 1% (w/v) n-octyl-beta-D-glucoside at 20°C Vibrio cholerae

Protein Variants

EC Number Protein Variants Comment Organism
3.3.2.1 D35N the mutation results in almost complete loss of enzyme activity Vibrio cholerae
3.3.2.1 H34A the mutant has about 80% of wild type activity Vibrio cholerae
3.3.2.1 H34N the mutant has about 35% of wild type activity Vibrio cholerae
3.3.2.1 K118A the mutant has about 30% of wild type activity Vibrio cholerae
3.3.2.1 K118H the mutation results in almost complete loss of enzyme activity Vibrio cholerae
3.3.2.1 K118N the mutation results in almost complete loss of enzyme activity Vibrio cholerae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.3.2.1 isochorismate + H2O Vibrio cholerae
-
(2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
-
?
3.3.2.1 isochorismate + H2O Vibrio cholerae N16961
-
(2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.1 Vibrio cholerae P0C6D3 O1 biovar El Tor
-
3.3.2.1 Vibrio cholerae N16961 P0C6D3 O1 biovar El Tor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.1 Ni2+ chelating Sepharose column chromatography and Superdex 200 gel filtration Vibrio cholerae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.1 isochorismate + H2O
-
Vibrio cholerae (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
-
?
3.3.2.1 isochorismate + H2O
-
Vibrio cholerae N16961 (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.3.2.1 ISC
-
Vibrio cholerae