BRENDA - Enzyme Database

The Saccharomyces cerevisiae enolase-related regions encode proteins that are active enolases

Kornblatt, M.J.; Richard Albert, J.; Mattie, S.; Zakaib, J.; Dayanandan, S.; Hanic-Joyce, P.J.; Joyce, P.B.; Yeast 30, 55-69 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
Mg2+
inhibitory at higher concentrations
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.03205
-
2-phospho-D-glycerate
isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
4.2.1.11
0.04482
-
2-phospho-D-glycerate
isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
4.2.1.11
0.05856
-
2-phospho-D-glycerate
isoform Err3p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
enolase is activated by low concentrations of Mg2+
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46672
-
x * 46672, isoform Eno1p, calculated from amino acid sequence
Saccharomyces cerevisiae
4.2.1.11
46673
-
x * 46673, isoform Eno1p, electrospray ionization mass spectrometry
Saccharomyces cerevisiae
4.2.1.11
47181
-
x * 47181, isoform Err3p, calculated from amino acid sequence
Saccharomyces cerevisiae
4.2.1.11
47184
-
x * 47184, isoform Err3p, electrospray ionization mass spectrometry
Saccharomyces cerevisiae
4.2.1.11
47196
-
x * 47196, isoform Err2p, calculated from amino acid sequence
Saccharomyces cerevisiae
4.2.1.11
47198
-
x * 47198, isoform Err2p, electrospray ionization mass spectrometry
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Saccharomyces cerevisiae
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
2-phospho-D-glycerate
Saccharomyces cerevisiae BY4743
-
phosphoenolpyruvate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Saccharomyces cerevisiae
-
-
-
4.2.1.11
Saccharomyces cerevisiae BY4743
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
Q-Sepharose column chromatography
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
731032
Saccharomyces cerevisiae
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
731032
Saccharomyces cerevisiae BY4743
phosphoenolpyruvate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 46672, isoform Eno1p, calculated from amino acid sequence; x * 46673, isoform Eno1p, electrospray ionization mass spectrometry; x * 47181, isoform Err3p, calculated from amino acid sequence; x * 47184, isoform Err3p, electrospray ionization mass spectrometry; x * 47196, isoform Err2p, calculated from amino acid sequence; x * 47198, isoform Err2p, electrospray ionization mass spectrometry
Saccharomyces cerevisiae
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.11
55.5
-
the melting temperature of isoform Eno1p, Err2, and Err3p are 55.5°C, 60.5°C, and 60°C, respectively
Saccharomyces cerevisiae
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.11
19.4
-
2-phospho-D-glycerate
isoform Err3p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
4.2.1.11
19.7
-
2-phospho-D-glycerate
isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
4.2.1.11
71.4
-
2-phospho-D-glycerate
isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.11
0.327
-
Mg2+
isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
4.2.1.11
0.695
-
Mg2+
isoform Err3p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
4.2.1.11
2.1
-
Mg2+
isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
Mg2+
inhibitory at higher concentrations
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.11
0.327
-
Mg2+
isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
4.2.1.11
0.695
-
Mg2+
isoform Err3p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
4.2.1.11
2.1
-
Mg2+
isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.03205
-
2-phospho-D-glycerate
isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
4.2.1.11
0.04482
-
2-phospho-D-glycerate
isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
4.2.1.11
0.05856
-
2-phospho-D-glycerate
isoform Err3p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
enolase is activated by low concentrations of Mg2+
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46672
-
x * 46672, isoform Eno1p, calculated from amino acid sequence
Saccharomyces cerevisiae
4.2.1.11
46673
-
x * 46673, isoform Eno1p, electrospray ionization mass spectrometry
Saccharomyces cerevisiae
4.2.1.11
47181
-
x * 47181, isoform Err3p, calculated from amino acid sequence
Saccharomyces cerevisiae
4.2.1.11
47184
-
x * 47184, isoform Err3p, electrospray ionization mass spectrometry
Saccharomyces cerevisiae
4.2.1.11
47196
-
x * 47196, isoform Err2p, calculated from amino acid sequence
Saccharomyces cerevisiae
4.2.1.11
47198
-
x * 47198, isoform Err2p, electrospray ionization mass spectrometry
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Saccharomyces cerevisiae
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
2-phospho-D-glycerate
Saccharomyces cerevisiae BY4743
-
phosphoenolpyruvate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
Q-Sepharose column chromatography
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
731032
Saccharomyces cerevisiae
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
731032
Saccharomyces cerevisiae BY4743
phosphoenolpyruvate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 46672, isoform Eno1p, calculated from amino acid sequence; x * 46673, isoform Eno1p, electrospray ionization mass spectrometry; x * 47181, isoform Err3p, calculated from amino acid sequence; x * 47184, isoform Err3p, electrospray ionization mass spectrometry; x * 47196, isoform Err2p, calculated from amino acid sequence; x * 47198, isoform Err2p, electrospray ionization mass spectrometry
Saccharomyces cerevisiae
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.11
55.5
-
the melting temperature of isoform Eno1p, Err2, and Err3p are 55.5°C, 60.5°C, and 60°C, respectively
Saccharomyces cerevisiae
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.11
19.4
-
2-phospho-D-glycerate
isoform Err3p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
4.2.1.11
19.7
-
2-phospho-D-glycerate
isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae
4.2.1.11
71.4
-
2-phospho-D-glycerate
isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication
Saccharomyces cerevisiae