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Literature summary extracted from
- Characterization of an extracellular thermophilic chitinase from Paenibacillus thermoaerophilus strain TC22-2b isolated from compost (2015), World J. Microbiol. Biotechnol., 31, 135-143.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | synthesis | the thermophilic chitinase may prove useful for industrial applications in chitooligosaccharide production from chitin | Paenibacillus thermoaerophilus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.14 | 1.4 | - |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside | pH 7.0, 60°C | Paenibacillus thermoaerophilus |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.14 | extracellular | - |
Paenibacillus thermoaerophilus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 48000 | - |
x * 48000, SDS-PAGE | Paenibacillus thermoaerophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Paenibacillus thermoaerophilus | - |
isolated from compost (55°C) in Japan | - |
| 3.2.1.14 | Paenibacillus thermoaerophilus TC22-2b | - |
isolated from compost (55°C) in Japan | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | native extracellular enzyme 4.5fold from cell culture medium by ammonium sulfate fractionation, colloidal chitin adsorption, and ion exchange chromatography | Paenibacillus thermoaerophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 1.32 | - |
purified enzyme, substrate 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside, pH 7.0, 50°C | Paenibacillus thermoaerophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Paenibacillus thermoaerophilus | 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
| 3.2.1.14 | 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Paenibacillus thermoaerophilus TC22-2b | 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
| 3.2.1.14 | colloidal chitin + H2O | prepared from commercial chitin | Paenibacillus thermoaerophilus | N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose | - |
? | |
| 3.2.1.14 | colloidal chitin + H2O | prepared from commercial chitin | Paenibacillus thermoaerophilus TC22-2b | N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose | - |
? | |
| 3.2.1.14 | additional information | the chitinase digests N-acetyl-chitooligosaccharides in an endo manner. N-acetylglucosamine dimers are not degraded by the enzyme | Paenibacillus thermoaerophilus | ? | - |
? | |
| 3.2.1.14 | additional information | the chitinase digests N-acetyl-chitooligosaccharides in an endo manner. N-acetylglucosamine dimers are not degraded by the enzyme | Paenibacillus thermoaerophilus TC22-2b | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | ? | x * 48000, SDS-PAGE | Paenibacillus thermoaerophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 60 | - |
- |
Paenibacillus thermoaerophilus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 30 | 70 | activity range | Paenibacillus thermoaerophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 50 | - |
the purified chitinase retains 68% of its initial activity after 2 h at 50°C | Paenibacillus thermoaerophilus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.14 | 9.6 | - |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside | pH 7.0, 60°C | Paenibacillus thermoaerophilus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 4 | - |
- |
Paenibacillus thermoaerophilus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 4 | 7 | 50% of maximal activity within this range | Paenibacillus thermoaerophilus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 3 | - |
purified enzyme, 30°C, 40% activity remaining after 2 h | Paenibacillus thermoaerophilus |
| 3.2.1.14 | 4 | 10 | purified enzyme, 30°C, 80% activity remaining after 2 h | Paenibacillus thermoaerophilus |
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Release 2023.1 (January 2023)
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