EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.26 | 2-mercaptoethanol | 134.4% activity at 1 mM | Aspergillus terreus | |
3.2.1.26 | NaBr | 130.9% activity at 1 mM | Aspergillus terreus | |
3.2.1.26 | NaCl | 120% activity at 1 mM | Aspergillus terreus | |
3.2.1.26 | NH4F | 130.5% activity at 1 mM | Aspergillus terreus | |
3.2.1.26 | Zn(NO3)2 | 155.4% activity at 1 mM | Aspergillus terreus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.26 | 2-mercaptoethanol | 87.8% residual activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | Ag+ | 18% residual activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | Al3+ | 20.5% residual activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | Co2+ | 63.5% residual activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | Cu2+ | 28.5% residual activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | EDTA | 47.9% residual activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | Fe2+ | 11.6% residual activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | Fe3+ | 16.5% residual activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | Hg+ | complete inhibition at 10 mM | Aspergillus terreus | |
3.2.1.26 | K+ | 67.1% residual activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | NaBr | 62.7% residual activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | NaH2PO4 | 67.9% residual activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | NH4Cl | 36.6% residual activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | NH4F | 51.3% residual activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | Zn(NO3)2 | 66.2% residual activity at 10 mM | Aspergillus terreus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.26 | 11.9 | - |
sucrose | in the presence of Mn2+, in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus | |
3.2.1.26 | 22.5 | - |
cellobiose | in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus | |
3.2.1.26 | 22.7 | - |
sucrose | in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus | |
3.2.1.26 | 123.6 | - |
cellobiose | in the presence of Mn2+, in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.26 | Ba2+ | 136% activity at 1 mM | Aspergillus terreus | |
3.2.1.26 | Ca2+ | 145% activity at 10 mM | Aspergillus terreus | |
3.2.1.26 | Co2+ | 168% activity at 1 mM | Aspergillus terreus | |
3.2.1.26 | Fe3+ | 117% activity at 1 mM | Aspergillus terreus | |
3.2.1.26 | K+ | 124% activity at 1 mM | Aspergillus terreus | |
3.2.1.26 | Mg2+ | 161% activity at 1 mM | Aspergillus terreus | |
3.2.1.26 | Mn2+ | 261% activity at 1 mM | Aspergillus terreus | |
3.2.1.26 | Zn2+ | 126% activity at 1 mM | Aspergillus terreus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 26900 | - |
1 * 46700 + 1 * 26900, SDS-PAGE | Aspergillus terreus |
3.2.1.26 | 46700 | - |
1 * 46700 + 1 * 26900, SDS-PAGE | Aspergillus terreus |
3.2.1.26 | 74800 | - |
gel filtration | Aspergillus terreus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.26 | Aspergillus terreus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.26 | DEAE-cellulose column chromatography and Sephacryl S-200 gel filtration | Aspergillus terreus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.26 | culture condition:rye flour-grown cell | - |
Aspergillus terreus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 3.9 | - |
crude extract, with sucrose as substrate, in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus |
3.2.1.26 | 555 | - |
after 139.4fold purification, with sucrose as substrate, in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.26 | cellobiose + H2O | - |
Aspergillus terreus | ? | - |
? | |
3.2.1.26 | inulin + H2O | worst substrate | Aspergillus terreus | ? | - |
? | |
3.2.1.26 | additional information | no activity with maltose, starch, and trehalose | Aspergillus terreus | ? | - |
? | |
3.2.1.26 | raffinose + H2O | - |
Aspergillus terreus | D-fructose + melibiose | - |
? | |
3.2.1.26 | sucrose + H2O | best substrate | Aspergillus terreus | D-fructose + D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.26 | heterodimer | 1 * 46700 + 1 * 26900, SDS-PAGE | Aspergillus terreus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.26 | invertase | - |
Aspergillus terreus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 55 | 65 | - |
Aspergillus terreus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 55 | 70 | the enzyme remains stable for 1 h at 55°C when diluted in water. The extracellular invertase is fully stable at lower temperatures, but half-lives of 40 and 5 min are estimated at 60 and 70°C, respectively | Aspergillus terreus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 4 | 6 | - |
Aspergillus terreus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 3 | 10 | the enzyme remains stable for 1 h | Aspergillus terreus |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.2.1.26 | 1 | - |
in 100 mM sodium acetate buffer, pH 5.0, at 50°C | Aspergillus terreus | Hg+ |