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Literature summary extracted from

  • Zhao, Y.; Meng, K.; Luo, H.; Huang, H.; Yuan, T.; Yang, P.; Yao, B.
    Molecular and biochemical characterization of a new alkaline active multidomain xylanase from alkaline wastewater sludge (2013), World J. Microbiol. Biotechnol., 29, 327-334.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expression in Escherichia coli uncultured microorganism

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 uncultured microorganism I6M1A2 isolated from alkaline wastewater sludge of a paper mill
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant protein uncultured microorganism

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 15.4
-
 substrate insoluble oat spelt xylan, pH 7.0, 60°C uncultured microorganism
3.2.1.8 26.6
-
 substrate insoluble wheat arabinoxylan xylan, pH 7.0, 60°C uncultured microorganism
3.2.1.8 41.2
-
 substrate beechwood xylan, pH 7.0, 60°C uncultured microorganism
3.2.1.8 55.1
-
 substrate soluble oat spelt xylan, pH 7.0, 60°C uncultured microorganism
3.2.1.8 63.5
-
 substrate birchwood xylan, pH 7.0, 60°C uncultured microorganism
3.2.1.8 67
-
 substrate soluble wheat arabinoxylan xylan, pH 7.0, 60°C uncultured microorganism

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beechwood xylan + H2O
-
 uncultured microorganism ?
-
 ?
3.2.1.8 birchwood xylan + H2O
-
 uncultured microorganism ?
-
 ?
3.2.1.8 insoluble oat spelt xylan + H2O
-
 uncultured microorganism ?
-
 ?
3.2.1.8 insoluble wheat arabinoxylan + H2O
-
 uncultured microorganism ?
-
 ?
3.2.1.8 soluble oat spelt xylan + H2O
-
 uncultured microorganism ?
-
 ?
3.2.1.8 soluble wheat arabinoxylan + H2O
-
 uncultured microorganism ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase A
-
 uncultured microorganism
3.2.1.8 Xyn-b39
-
 uncultured microorganism

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 60
-
-
 uncultured microorganism

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 55
-
 2 h, 90% residual activity uncultured microorganism

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 7
-
-
 uncultured microorganism

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 9
-
 80% of maximum acivity uncultured microorganism
3.2.1.8 10
-
 40% of maximum activity uncultured microorganism