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Literature summary extracted from

  • Prakash, P.; Jayalakshmi, S.K.; Prakash, B.; Rubul, M.; Sreeramulu, K.
    Production of alkaliphilic, halotolerent, thermostable cellulase free xylanase by Bacillus halodurans PPKS-2 using agro waste: single step purification and characterization (2012), World J. Microbiol. Biotechnol., 28, 183-192.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 EDTA 1 mM, 114% of initial activity Halalkalibacterium halodurans

Application

EC Number Application Comment Organism
3.2.1.8 synthesis yield of enzyme is enhanced more than four fold in the presence of 1% corn husk and 0.5% peptone or feather hydrolysate at pH 11 and 37°C Halalkalibacterium halodurans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 dithiothreitol 1 mM, 65% residual activity Halalkalibacterium halodurans
3.2.1.8 Hg2+ 1 mM, 10% residual activity Halalkalibacterium halodurans
3.2.1.8 Mn2+ 1 mM, 4.8% residual activity Halalkalibacterium halodurans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 oat spelt xylan Km value is 4.2 mg/ml, pH 11, 70°C Halalkalibacterium halodurans
3.2.1.8 additional information
-
 birchwood xylan Km value is 4.4 mg/ml, pH 11, 70°C Halalkalibacterium halodurans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Halalkalibacterium halodurans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 24000
-
 x * 24000, SDS-PAGE Halalkalibacterium halodurans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Halalkalibacterium halodurans
-
-
-
3.2.1.8 Halalkalibacterium halodurans PPKS-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Halalkalibacterium halodurans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 214
-
 pH 11, 70°C Halalkalibacterium halodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O
-
 Halalkalibacterium halodurans D-xylose + ?
-
 ?
3.2.1.8 birchwood xylan + H2O
-
 Halalkalibacterium halodurans PPKS-2 D-xylose + ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Halalkalibacterium halodurans D-xylose + ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Halalkalibacterium halodurans PPKS-2 D-xylose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 24000, SDS-PAGE Halalkalibacterium halodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 70
-
-
 Halalkalibacterium halodurans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 80
-
 80% of maximum activity Halalkalibacterium halodurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 100
-
 3 h, no loss of activity Halalkalibacterium halodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 11
-
-
 Halalkalibacterium halodurans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 10 12 more than 75% of maximum activity within Halalkalibacterium halodurans