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Literature summary extracted from

  • Du, J.; Zhou, Y.; Su, X.; Yu, J.J.; Khan, S.; Jiang, H.; Kim, J.; Woo, J.; Kim, J.H.; Choi, B.H.; He, B.; Chen, W.; Zhang, S.; Cerione, R.A.; Auwerx, J.; Hao, Q.; Lin, H.
    Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase (2011), Science, 334, 806-809.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.B43 crystal structure of Sirt5 in complex with a thioacetyl peptide is obtained, The corresponding acetyl peptide could not be crystallized with Sirt5 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.B43 H158Y mutation significantly increases the Km for desuccinylation Homo sapiens
2.3.1.B43 R105M mutation significantly increases the Km for desuccinylation Homo sapiens
2.3.1.B43 Y102F mutation significantly increases the Km for desuccinylation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.B43 0.0058
-
 KQTAR(N6-malonyl)KSTGGWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 0.0061
-
 KQTAR(N6-succinyl)KSTGGWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 0.0087
-
 SGASE(N6-succinyl)KDIVHSGWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 0.014
-
 SGASE(N6-malonyl)KDIVHSGWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 0.15
-
 KTRSG(N6-succinyl)KVMRRWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 0.45
-
 KTRSG(N6-malonyl)KVMRRWW pH 7.5, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.B43 NAD+ + [carbamoyl phosphate synthase 1]-N6-succinyl-L-lysine Mus musculus deletion of Sirt5 in mice increases the level of succinylation on carbamoyl phosphate synthase 1 nicotinamide + [carbamoyl phosphate synthase 1]-L-lysine + 2'-O-succinyl-ADP ribose
-
 ?
2.3.1.B43 NAD+ + [protein]-N6-malonyl-L-lysine Homo sapiens
-
 nicotinamide + [protein]-L-lysine + 2'-O-malonyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + [protein]-N6-succinyl-L-lysine Homo sapiens protein lysine succinylation may represent a posttranslational modification that can be reversed by Sirt5 in vivo. Lysine succinylation occurs on several mammalian proteins nicotinamide + [protein]-L-lysine + 2'-O-succinyl-ADP-ribose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.B43 Homo sapiens Q9NXA8
-
-
2.3.1.B43 Mus musculus Q8K2C6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.B43 additional information catalytic efficiency (kcat/Km) of Sirt5 with the histone H3K9 acetyl peptide is about 500fold lower than that of Sirt1. Sirtuin 5 has only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr102 and Arg105) that bind to malonylated and succinylated substrates and define the specificity Homo sapiens ?
-
 ?
2.3.1.B43 NAD+ + KQTAR(N6-malonyl)KSTGGWW histone H3K9 malonyl peptide Homo sapiens nicotinamide + KQTARKSTGGWW + 2'-O-malonyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + KQTAR(N6-succinyl)KSTGGWW histone H3K9 succinyl peptide Homo sapiens nicotinamide + KQTARKSTGGWW + 2'-O-succinyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + KTRSG(N6-malonyl)KVMRRWW
-
 Homo sapiens nicotinamide + KTRSGKVMRRWW + 2'-O-malonyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + KTRSG(N6-succinyl)KVMRRWW ACS2 Lys628 peptide Homo sapiens nicotinamide + KTRSGKVMRRWW + 2'-O-succinyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + SGASE(N6-malonyl)KDIVHSGWW
-
 Homo sapiens nicotinamide + SGASEKDIVHSGWW + 2'-O-malonyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + SGASE(N6-succinyl)KDIVHSGWW glutamate dehydrogenase Lys503 peptide Homo sapiens nicotinamide + SGASEKDIVHSGWW + 2'-O-succinyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + [carbamoyl phosphate synthase 1]-N6-succinyl-L-lysine deletion of Sirt5 in mice increases the level of succinylation on carbamoyl phosphate synthase 1 Mus musculus nicotinamide + [carbamoyl phosphate synthase 1]-L-lysine + 2'-O-succinyl-ADP ribose
-
 ?
2.3.1.B43 NAD+ + [protein]-N6-malonyl-L-lysine
-
 Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-malonyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + [protein]-N6-succinyl-L-lysine protein lysine succinylation may represent a posttranslational modification that can be reversed by Sirt5 in vivo. Lysine succinylation occurs on several mammalian proteins Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-succinyl-ADP-ribose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.B43 SIRT5
-
 Homo sapiens
2.3.1.B43 SIRT5
-
 Mus musculus
2.3.1.B43 sirtuin 5
-
 Homo sapiens
2.3.1.B43 sirtuin 5
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.B43 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.B43 0.014
-
 SGASE(N6-succinyl)KDIVHSGWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 0.025
-
 KQTAR(N6-malonyl)KSTGGWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 0.028
-
 SGASE(N6-malonyl)KDIVHSGWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 0.037
-
 KQTAR(N6-succinyl)KSTGGWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 0.079
-
 KTRSG(N6-succinyl)KVMRRWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 0.268
-
 KTRSG(N6-malonyl)KVMRRWW pH 7.5, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.B43 7.5
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.B43 malfunction deletion of Sirt5 in mice increases the level of succinylation on carbamoyl phosphate synthase 1 Mus musculus
2.3.1.B43 physiological function protein lysine succinylation may represent a posttranslational modification that can be reversed by Sirt5 Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.B43 0.52
-
 KTRSG(N6-succinyl)KVMRRWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 0.6
-
 KTRSG(N6-malonyl)KVMRRWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 1.6
-
 SGASE(N6-succinyl)KDIVHSGWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 2
-
 SGASE(N6-malonyl)KDIVHSGWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 4.3
-
 KQTAR(N6-malonyl)KSTGGWW pH 7.5, 37°C Homo sapiens
2.3.1.B43 6.1
-
 KQTAR(N6-succinyl)KSTGGWW pH 7.5, 37°C Homo sapiens