BRENDA - Enzyme Database

Molecular cloning, expression and characterization of enolase from adult Haemonchus contortus

Han, K.; Xu, L.; Yan, R.; Song, X.; Li, X.; Res. Vet. Sci. 92, 259-265 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Haemonchus contortus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
extracellular
-
Haemonchus contortus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47074
-
x * 47074, calculated from amino acid sequence
Haemonchus contortus
4.2.1.11
49000
-
x * 49000, SDS-PAGE
Haemonchus contortus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Haemonchus contortus
-
phosphoenolpyruvate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Haemonchus contortus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA column chromatography
Haemonchus contortus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
730926
Haemonchus contortus
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
3-phospho-D-erythronate
-
730926
Haemonchus contortus
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47074, calculated from amino acid sequence; x * 49000, SDS-PAGE
Haemonchus contortus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7
-
-
Haemonchus contortus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.2.1.11
6
8
remains stable between pH 6.0 and 8.0
Haemonchus contortus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Haemonchus contortus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
extracellular
-
Haemonchus contortus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47074
-
x * 47074, calculated from amino acid sequence
Haemonchus contortus
4.2.1.11
49000
-
x * 49000, SDS-PAGE
Haemonchus contortus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Haemonchus contortus
-
phosphoenolpyruvate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA column chromatography
Haemonchus contortus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
730926
Haemonchus contortus
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
3-phospho-D-erythronate
-
730926
Haemonchus contortus
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47074, calculated from amino acid sequence; x * 49000, SDS-PAGE
Haemonchus contortus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7
-
-
Haemonchus contortus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.2.1.11
6
8
remains stable between pH 6.0 and 8.0
Haemonchus contortus