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Literature summary extracted from

  • Kusuma, M.; Sri Rami Reddy, D.
    Purification and characterization of polygalacturonase using isolated Bacillus subtilis C4 (2014), Res. J. Microbiol., 9, 95-103.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.15 ascorbic acid
-
 Bacillus subtilis
3.2.1.15 L-cysteine
-
 Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 Hg2+
-
 Bacillus subtilis
3.2.1.15 Mn2+
-
 Bacillus subtilis
3.2.1.15 Zn2+
-
 Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.15 extracellular
-
 Bacillus subtilis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.15 Ca2+ activates slightly at 10 mM Bacillus subtilis
3.2.1.15 Mg2+ activates slightly at 10 mM Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Bacillus subtilis
-
-
-
3.2.1.15 Bacillus subtilis C4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 native enzyme 15fold by ammonium sulfate fractionation, dialysis, anion exchange and affinity chromatography Bacillus subtilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.15 additional information submerged fermentation on pectin Bacillus subtilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 13790
-
 purified enzyme, pH 9.0, 60°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 amylopectin + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.15 amylopectin + H2O
-
 Bacillus subtilis C4 ?
-
 ?
3.2.1.15 dextrose agar + H2O potato dextrose agar Bacillus subtilis ?
-
 ?
3.2.1.15 dextrose agar + H2O potato dextrose agar Bacillus subtilis C4 ?
-
 ?
3.2.1.15 pectin + H2O high activity Bacillus subtilis ?
-
 ?
3.2.1.15 pectin + H2O high activity Bacillus subtilis C4 ?
-
 ?
3.2.1.15 polygalacturonic acid + H2O best substrate Bacillus subtilis galacturonic acid
-
 ?
3.2.1.15 polygalacturonic acid + H2O best substrate Bacillus subtilis C4 galacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 ? x * 43000-66000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 alkaline endo-PG
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 60
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.15 20 90 activity range Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 40 50 purified enzyme, pH 9.0, 60 min, stable Bacillus subtilis
3.2.1.15 60
-
 purified enzyme, pH 9.0, 60 min, loss of 80% activity Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 9
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.15 3 10 activity range Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.15 9
-
 purified enzyme, 60°C, 60 min, stable Bacillus subtilis