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Literature summary extracted from

  • Matoba, Y.; Tanaka, N.; Noda, M.; Higashikawa, F.; Kumagai, T.; Sugiyama, M.
    Crystallographic and mutational analyses of tannase from Lactobacillus plantarum (2013), Proteins, 81, 2052-2058.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.20 expressed in Escherichia coli BL21(DE3) and B834(DE3) cells Lactiplantibacillus plantarum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.20 sitting drop vapor diffusion method, using 0.1 M sodium acetate buffer (pH 5.0) containing 15-20% (w/v) polyethylene glycol 8000 and 0.2 M ammonium sulfate Lactiplantibacillus plantarum

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.20 D421A the mutant has 44fold lower activity than the wild type enzyme Lactiplantibacillus plantarum
3.1.1.20 E357A the mutant has 230fold lower activity than the wild type enzyme Lactiplantibacillus plantarum
3.1.1.20 K343A the mutant has very little activity compared to the wild type enzyme Lactiplantibacillus plantarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 0.62
-
methyl gallate wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 0.95
-
methyl gallate mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 1
-
methyl gallate mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Lactiplantibacillus plantarum
-
gallate + D-glucose
-
?
3.1.1.20 tannic acid + H2O Lactiplantibacillus plantarum SN35N
-
gallate + D-glucose
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Lactiplantibacillus plantarum B3Y018
-
-
3.1.1.20 Lactiplantibacillus plantarum SN35N B3Y018
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 Ni2+ affinity column chromatography Lactiplantibacillus plantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 methyl gallate + H2O
-
Lactiplantibacillus plantarum gallate + methanol
-
?
3.1.1.20 methyl gallate + H2O
-
Lactiplantibacillus plantarum SN35N gallate + methanol
-
?
3.1.1.20 tannic acid + H2O
-
Lactiplantibacillus plantarum gallate + D-glucose
-
?
3.1.1.20 tannic acid + H2O
-
Lactiplantibacillus plantarum SN35N gallate + D-glucose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannin acylhydrolase
-
Lactiplantibacillus plantarum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.20 0.53
-
methyl gallate mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 2.5
-
methyl gallate mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 74
-
methyl gallate wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.20 0.021
-
methyl gallate mutant enzyme K343A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 0.52
-
methyl gallate mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 2.7
-
methyl gallate mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 120
-
methyl gallate wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum