EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.20 | expressed in Escherichia coli BL21(DE3) and B834(DE3) cells | Lactiplantibacillus plantarum |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.1.20 | sitting drop vapor diffusion method, using 0.1 M sodium acetate buffer (pH 5.0) containing 15-20% (w/v) polyethylene glycol 8000 and 0.2 M ammonium sulfate | Lactiplantibacillus plantarum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.1.20 | D421A | the mutant has 44fold lower activity than the wild type enzyme | Lactiplantibacillus plantarum |
3.1.1.20 | E357A | the mutant has 230fold lower activity than the wild type enzyme | Lactiplantibacillus plantarum |
3.1.1.20 | K343A | the mutant has very little activity compared to the wild type enzyme | Lactiplantibacillus plantarum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.20 | 0.62 | - |
methyl gallate | wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
3.1.1.20 | 0.95 | - |
methyl gallate | mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
3.1.1.20 | 1 | - |
methyl gallate | mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.20 | tannic acid + H2O | Lactiplantibacillus plantarum | - |
gallate + D-glucose | - |
? | |
3.1.1.20 | tannic acid + H2O | Lactiplantibacillus plantarum SN35N | - |
gallate + D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.20 | Lactiplantibacillus plantarum | B3Y018 | - |
- |
3.1.1.20 | Lactiplantibacillus plantarum SN35N | B3Y018 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.20 | Ni2+ affinity column chromatography | Lactiplantibacillus plantarum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.20 | methyl gallate + H2O | - |
Lactiplantibacillus plantarum | gallate + methanol | - |
? | |
3.1.1.20 | methyl gallate + H2O | - |
Lactiplantibacillus plantarum SN35N | gallate + methanol | - |
? | |
3.1.1.20 | tannic acid + H2O | - |
Lactiplantibacillus plantarum | gallate + D-glucose | - |
? | |
3.1.1.20 | tannic acid + H2O | - |
Lactiplantibacillus plantarum SN35N | gallate + D-glucose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.20 | tannin acylhydrolase | - |
Lactiplantibacillus plantarum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.20 | 0.53 | - |
methyl gallate | mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
3.1.1.20 | 2.5 | - |
methyl gallate | mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
3.1.1.20 | 74 | - |
methyl gallate | wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.20 | 0.021 | - |
methyl gallate | mutant enzyme K343A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
3.1.1.20 | 0.52 | - |
methyl gallate | mutant enzyme E357A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
3.1.1.20 | 2.7 | - |
methyl gallate | mutant enzyme D421A, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
3.1.1.20 | 120 | - |
methyl gallate | wild type enzyme, in 25 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum |